{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Yoichiro Sugimoto"],"instrument_platform":["Illumina NovaSeq 6000"],"study_type":["RNA-seq of coding RNA"],"organism":["Ovis aries"],"species":["Ovis aries"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-17285"],"description":["Bulk RNA sequencing of carotid body (CB) and superior cervical ganglion (SCG) tissues from sheep at four developmental stages: fetal day 120, fetal day 145 (term), post-natal day 15, and adult. Three biological replicates were collected per tissue per stage (one d145 SCG sample was excluded for poor RNA quality and low sympathetic-marker expression in the analysis). The study aimed to identify CB-specific, developmentally regulated gene expression changes correlating with the peri-natal maturation of oxygen chemosensitivity."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Library Construction - The integrity of RNA was assessed by Agilent Bioanalyzer. Strand-specific RNA-seq libraries were prepared using the KAPA RNA HyperPrep Kit with RiboErase (Roche), which depletes ribosomal RNA prior to library construction. Libraries were quality-checked and quantified before pooling.","Sequencing - Pooled, indexed libraries were sequenced on an Illumina NovaSeq platform in paired-end mode. Base calling and demultiplexing were performed using Illumina's standard pipeline.","Sample Collection - Tissues were dissected as described in (https://www.biorxiv.org/cgi/content/short/2025.08.21.671502v1) and snap-frozen in liquid nitrogen. The frozen samples were lysed in RNA lysis buffer (Qiagen) and refined to a fine suspension using a hand-held motorised homogeniser with a 5 mm probe (Pro-Scientific).","Nucleic Acid Extraction - RNA extraction was performed using the RNA Clean & Concentrator-5 kit (Zymo)."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Yoichiro Sugimoto"],"additional_accession":[]},"is_claimable":false,"name":"mRNA profiling of the sheep carotid body and superior cervical ganglion across development","description":"Bulk RNA sequencing of carotid body (CB) and superior cervical ganglion (SCG) tissues from sheep at four developmental stages: fetal day 120, fetal day 145 (term), post-natal day 15, and adult. Three biological replicates were collected per tissue per stage (one d145 SCG sample was excluded for poor RNA quality and low sympathetic-marker expression in the analysis). The study aimed to identify CB-specific, developmentally regulated gene expression changes correlating with the peri-natal maturation of oxygen chemosensitivity.","dates":{"release":"2026-07-07T00:00:00Z","modification":"2026-07-07T09:00:27.685Z","creation":"2026-07-06T13:29:03.442Z"},"accession":"E-MTAB-17285","cross_references":{"ENA":["ERP196214"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005518","EFO_0003738","EFO_0004184"]}}