biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsYoichiro SugimotoIllumina NovaSeq 6000RNA-seq of coding RNAOvis ariesOvis arieshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-17285Bulk RNA sequencing of carotid body (CB) and superior cervical ganglion (SCG) tissues from sheep at four developmental stages: fetal day 120, fetal day 145 (term), post-natal day 15, and adult. Three biological replicates were collected per tissue per stage (one d145 SCG sample was excluded for poor RNA quality and low sympathetic-marker expression in the analysis). The study aimed to identify CB-specific, developmentally regulated gene expression changes correlating with the peri-natal maturation of oxygen chemosensitivity.biostudies-arrayexpressLibrary Construction - The integrity of RNA was assessed by Agilent Bioanalyzer. Strand-specific RNA-seq libraries were prepared using the KAPA RNA HyperPrep Kit with RiboErase (Roche), which depletes ribosomal RNA prior to library construction. Libraries were quality-checked and quantified before pooling.Sequencing - Pooled, indexed libraries were sequenced on an Illumina NovaSeq platform in paired-end mode. Base calling and demultiplexing were performed using Illumina's standard pipeline.Sample Collection - Tissues were dissected as described in (https://www.biorxiv.org/cgi/content/short/2025.08.21.671502v1) and snap-frozen in liquid nitrogen. The frozen samples were lysed in RNA lysis buffer (Qiagen) and refined to a fine suspension using a hand-held motorised homogeniser with a 5 mm probe (Pro-Scientific).Nucleic Acid Extraction - RNA extraction was performed using the RNA Clean & Concentrator-5 kit (Zymo).OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesYoichiro SugimotofalsemRNA profiling of the sheep carotid body and superior cervical ganglion across developmentBulk RNA sequencing of carotid body (CB) and superior cervical ganglion (SCG) tissues from sheep at four developmental stages: fetal day 120, fetal day 145 (term), post-natal day 15, and adult. Three biological replicates were collected per tissue per stage (one d145 SCG sample was excluded for poor RNA quality and low sympathetic-marker expression in the analysis). The study aimed to identify CB-specific, developmentally regulated gene expression changes correlating with the peri-natal maturation of oxygen chemosensitivity.2026-07-07T00:00:00Z2026-07-07T09:00:27.685Z2026-07-06T13:29:03.442ZE-MTAB-17285ERP196214EFO_0002944EFO_0004170EFO_0005518EFO_0003738EFO_0004184