{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Nicole Leonie Bertschi"],"organism":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-17299"],"description":["Effect of Tapinaorf on human mTh1, mTh2 and mTh17. mTh1, mTh2 and mTh17 cells were e treated with tapinarof (10uM) or DMSO (control) for 24 hours either in the resting state or after activation with aCD3/CD2/CD28."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Growth Protocol - Culture medium consisted of PRMI-1640 with Hepes (GIBCO) supplemented with 5% of heat-inactivated human serum (Swiss Red Cross, Basel, Switzerland), 2mM L-Glutamine (Biochrom), 50U/ml penicillin and 50μg/ml streptomycin (Bioconcept) and 50 IU/ml IL-2 (Roche). T cells were cultured at a density of 0.25-1x105cells/96 well plate in a total volume of 200μL cell culture medium","Nucleic Acid Extraction - Total RNA was isolated with RNAeasy kit from Qiagen as per the manufacturer’s instructions","Library Construction - Illumina TruSeq Stranded mRNA protocol (Directional, first strand)","Sequencing - Single-end 100 bp sequencing by Next Generation Sequencing Platform of the University of Bern","Sample Treatment - mTh1, mTh2 and Th17 cells were incubated with Tapinarof for 24 hours either in the resting state or activated with aCD3/2/28.","Sample Collection - Peripheral Blood Mononuclear Cells (PBMC) were isolated by Ficoll-Plaque Plus (GE Healthcare, UK) density gradient centrifugation. CD4+ T cells were isolated from PBMCs using the EasySep positive selection kit (Cat. #17852, Stemcell Technologies) as per the manufacturer’s instructions. Positively selected CD4+ T cells were stained for the subsequent sorting of the TH cell subset. Memory TH cell subsets were sorted with a purity of > 90% according to the expression of chemokine receptors from CD45RA-CD25-CD8-CD3+ cells: TH1 (CXCR3+CCR6-CCR4-), TH2 (CXCR3-CCR6-CCR4+) and TH17 (CXCR3-CCR6+CCR4+)."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"data_protocol":["Data Transformation - RNA-seq reads were mapped to the human reference genome (GRCh38, build 81) using HISAT2 v. 2.0.4 (Kim et al., 2015). HTseq-count v. 0.6.1 (Anders et al., 2015) to count the number of reads per gene, and DESeq2 v.1.4.5 (Love et al., 2014) to test for differential expression between groups of samples was used."],"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["Illumina NovaSeq 6000"],"study_type":["RNA-seq of coding RNA"],"species":["Homo sapiens"],"pubmed_authors":["Nicole Leonie Bertschi"],"additional_accession":[]},"is_claimable":false,"name":"RNA-seq of human memory Th1 (mTh1), mTh2 and mTh17 cells treated with tapinarof against untreated control","description":"Effect of Tapinaorf on human mTh1, mTh2 and mTh17. mTh1, mTh2 and mTh17 cells were e treated with tapinarof (10uM) or DMSO (control) for 24 hours either in the resting state or after activation with aCD3/CD2/CD28.","dates":{"release":"2026-06-28T00:00:00Z","modification":"2026-07-09T12:21:03.523Z","creation":"2026-07-09T11:54:48.566Z"},"accession":"E-MTAB-17299","cross_references":{"ENA":["ERP200976"],"EFO":["EFO_0002944","EFO_0004170","EFO_0003789","EFO_0005518","EFO_0003816","EFO_0003738","EFO_0004184","EFO_0003969"]}}