biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsSong GaoIllumina NovaSeq 6000RNA-seq of coding RNAHomo sapiensHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-17303To investigate the transcriptomic changes associated with ARDS progression, we performed RNA-seq on PBMCs from 6 healthy donors (Con), 7 ARDS patientsbiostudies-arrayexpressNucleic Acid Extraction - Total RNA was extracted from PBMCs using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's instructions. RNA quality and quantity were assessed using a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA) and agarose gel electrophoresis. RNA sequencing libraries were constructed using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, Ipswich, MA, USA) and sequenced on the Illumina NovaSeq 6000 platform (Illumina, San Diego, CA, USA) with 150-bp paired-end reads.Sequencing - Raw sequencing reads were filtered to remove low-quality reads, adapter sequences, and reads containing poly-N sequences using FastQC (v0.11.9) and Trimmomatic (v0.39). Clean reads were aligned to the human reference genome (GRCh38) using HISAT2 (v2.2.1).Sample Collection - Peripheral blood samples were collected from 6 healthy volunteers (Con group), 7 patients meeting the Berlin Definition of ARDS at the Intensive Care Units of Xishan People's Hospital of Wuxi City and Affiliated Hospital of Jiangnan University between January 2024 and December 2024.Library Construction - Total RNA was extracted from PBMCs using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's instructions. RNA quality and quantity were assessed using a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA) and agarose gel electrophoresis. RNA sequencing libraries were constructed using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, Ipswich, MA, USA) and sequenced on the Illumina NovaSeq 6000 platform (Illumina, San Diego, CA, USA) with 150-bp paired-end reads.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesSong GaofalseTranscriptomic analysis identifies metabolic dysregulation in ARDS patientsTo investigate the transcriptomic changes associated with ARDS progression, we performed RNA-seq on PBMCs from 6 healthy donors (Con), 7 ARDS patients2026-06-30T00:00:00Z2026-07-03T14:19:49.498Z2026-07-03T14:19:32.025ZE-MTAB-17303ERP196147EFO_0002944EFO_0004170EFO_0005518EFO_0003738EFO_0004184