biostudies-arrayexpressMarek MutwilArabidopsis thalianaKallistohttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-17310This experiment seeks to elucidate the functional role of DREB2G in Arabidopsis thaliana seeds via differential gene expression (DGE) analysis. Total RNA were extracted from pooled Arabidopsis thaliana seeds at 10-2 days after flowering (DAF) for 3 biological replicates from WT and 2 independent DREB2G OE lines. RNA-seq libraries were generated using NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs) for Illumina according to the manufacturer’s instruction and sequenced with Novaseq-6000 (Illumina) using paired-end sequencing with read lengths of 150 base pairs at sequencing depths of ~ 2 million reads per sample.biostudies-arrayexpressGrowth Protocol - Arabidopsis thaliana seedlings are grown chambers under constant temperature of 23°C and a photoperiod of 16 h light (100‐150 μmol m−2 sec−1 354 illumination)/8 h dark.Sequencing - Paired-end sequencing was conducted using Novaseq-6000 (Illumina) with read lengths of 150 base pairs at sequencing depths of ~ 2 million reads per sampleSample Collection - Total RNA were extracted from pooled Arabidopsis thaliana seeds 10-12 days after flowering (DAF).Library Construction - RNA-seq libraries were generated using NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs) for Illumina according to the manufacturer’s instructionNucleic Acid Extraction - Total RNA extraction was carried out using the Monarch Total RNA Miniprep Kit (New England Biolabs), following the manufacturer’s protocols. Subsequently, DNase I (New England Biolabs) was employed to remove genomic DNA, according to the manufacturer's instructions.OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesSequence Alignment - Coding sequences of the Arabidopsis thaliana nuclear genome (Araport 11) from The Arabidopsis Information Resource database (https://www.arabidopsis.org) were used as read pseudo alignment references using Kallisto to estimate gene expression (est. counts).Data Transformation - Gene expression abundances of samples are provided as estimated counts (est. counts) and Transcripts Per Million (TPM) in counts_matrix.txt and TPM_matrix.txt, respectively. These two metrics are reported by Kallisto pseudoaligner.MetabolomicsUnknownTranscriptomicsGenomicsProteomicsIllumina NovaSeq 6000RNA-seq of coding RNAArabidopsis thalianaMarek MutwilPeng Ken LimfalseRNA-seq of Arabidopsis thaliana seeds from WT and DREB2G overexpression (OE) linesThis experiment seeks to elucidate the functional role of DREB2G in Arabidopsis thaliana seeds via differential gene expression (DGE) analysis. Total RNA were extracted from pooled Arabidopsis thaliana seeds at 10-2 days after flowering (DAF) for 3 biological replicates from WT and 2 independent DREB2G OE lines. RNA-seq libraries were generated using NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs) for Illumina according to the manufacturer’s instruction and sequenced with Novaseq-6000 (Illumina) using paired-end sequencing with read lengths of 150 base pairs at sequencing depths of ~ 2 million reads per sample.2026-07-20T00:00:00Z2026-07-20T01:00:49.779Z2026-07-10T14:40:15.853ZE-MTAB-17310ERP201041EFO_0002944EFO_0004170EFO_0003789EFO_0004917EFO_0005518EFO_0003816EFO_0003738EFO_0004184