biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsTodsapol TechoIllumina HiSeq 2500RNA-seq of coding RNASaccharomyces cerevisiae S288CSaccharomyces cerevisiae S288Chttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-17319RNA-seq was performed to characterize the transcriptional response of S. cerevisiae BY4742 to sub-inhibitory gallic acid (GA) treatment (1 mg/mL, 16 h) versus untreated control, to identify genes involved in copper/iron homeostasis and antioxidant defense.biostudies-arrayexpressLibrary Construction - NEBNext® Ultra™ RNA Library Prep Kit for Illumina®Sample Collection - Cells from two biological replicates were harvested by centrifugation and used for RNA extraction.Growth Protocol - YPD broth, 30°C, 200 rpm, initial OD₆₀₀ 0.003, 16 hSequencing - Illumina HiSeq and 2 × 150 bp paired-end (PE150)Sample Treatment - ± 1 mg mL⁻¹ gallic acidNucleic Acid Extraction - Total RNA was isolated using the FavorPrep Tissue Total RNA Purification Mini Kit (Favorgen), following the manufacturer’s protocol.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesTodsapol TechofalseGallic acid modulates copper and iron homeostasis and antioxidant systems in Saccharomyces cerevisiaeRNA-seq was performed to characterize the transcriptional response of S. cerevisiae BY4742 to sub-inhibitory gallic acid (GA) treatment (1 mg/mL, 16 h) versus untreated control, to identify genes involved in copper/iron homeostasis and antioxidant defense.2026-08-28T00:00:00Z2026-08-28T11:17:04.821Z2026-07-15T21:34:23.055ZE-MTAB-17319ERP201290EFO_0002944EFO_0004170EFO_0003789EFO_0005518EFO_0003738EFO_0004184EFO_0003969