{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Nicole Leonie Bertschi"],"instrument_platform":["Illumina NovaSeq 6000"],"study_type":["RNA-seq of coding RNA"],"organism":["Homo sapiens"],"species":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-17321"],"description":["Effect of topical Tapinarof (VTAMA) on human Genoskin skin explants. Human Genoskin skin explants were treated with VTAMA(1% tapinarof) or Vehicle (control) for 2 and 5 days."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sample Collection - Skin explants were transferred immediately into culture medium. Prior to experimental manipulation, explants were equilibrated for at least 1 h at 37 °C in a humidified atmosphere containing 5% CO₂. Explants were cultured in 12-well plates for up to 5 days in a proprietary, chemically defined, hydrocortisone- and serum-free medium supplemented with penicillin (100 µg/mL) and streptomycin (100 µg/mL). Cultures were maintained at 37 °C and 5% CO₂, and culture medium was replaced daily. Skin explants were treated topically once daily with either vehicle control (Laboratoire Gola, Galenicare SA) or VTAMA (tapinarof cream, 1%, Dermavant Sciences) throughout the culture period.","Library Construction - Illumina TruSeq Stranded mRNA protocol (Directional, first strand)","Sequencing - The mRNA was sequenced by the Next Generation Sequencing Platform of Bern using paired-end 100 bp sequencing.","Nucleic Acid Extraction - Total RNA from Genoskin samples was isolated using the RNeasy Lipid Tissue Kit (Cat. 74804, Qiagen) according to the manufacturer’s instructions."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Nicole Leonie Bertschi"],"additional_accession":[]},"is_claimable":false,"name":"RNA-seq of human Genoskin explants treated with topical VTAMA (tapinarof) against untreated control","description":"Effect of topical Tapinarof (VTAMA) on human Genoskin skin explants. Human Genoskin skin explants were treated with VTAMA(1% tapinarof) or Vehicle (control) for 2 and 5 days.","dates":{"release":"2026-07-26T00:00:00Z","modification":"2026-07-26T01:00:49.512Z","creation":"2026-07-15T21:37:19.557Z"},"accession":"E-MTAB-17321","cross_references":{"ENA":["ERP201291"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005518","EFO_0003738","EFO_0004184"]}}