{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Jianyao Xue"],"organism":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-17338"],"description":["Acute hemolysis can deplete haptoglobin and hemopexin, leaving toxic free heme insufficiently controlled. Here, the authors show that scavenger receptor BI (SR-BI) mediates hepatic uptake of free heme and is essential for protection against heme-induced injury and death. Here we examined gene expression post hemin injections"],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sequencing - (1) Data quality control: filtering reads containing adapter or with low quality; (2) Statistics analysis of data production of quality; (3) Mapping reads to reference genome (STAR); (4) Gene expression quantification (HTseq); (5) Differential expression analysis (DEseq2/edgeR). pair-end reads were 150-base-pair ones, output is 9G. NovaSeq 6000 Illumina platform was used","Nucleic Acid Extraction - Total RNA was isolated by using RNeasy mini kit from Qiagen","Sample Collection - SR-BIfl/fl and AlbcreSR-BIfl/fl mice were treated with/without hemin at 40 µmol/kg BW, i.p. Twenty-four hours later, a second injection was performed. Thirty-eight hours post the initial injection, RNA was isolated from Kidney, lung and liver using Qiagen RNeasy Mini Kit, and sent to Novogene for RNA sequencing analysis.","Library Construction - Total RNA was sent to Novogene for library construction"],"figure_sub":["Organization","MINSEQE Score","Assays and Data","Processed Data","MAGE-TAB Files"],"data_protocol":["Data Transformation - (1) Data quality control: filtering reads containing adapter or with low quality; (2) Statistics analysis of data production of quality; (3) Mapping reads to reference genome (STAR); (4) Gene expression quantification (HTseq); (5) Differential expression analysis (DEseq2/edgeR). pair-end reads were 150-base-pair ones, output is 9G. NovaSeq 6000 Illumina platform was used Supplementary files format and content: fpkm Supplementary files format and content: readcount"],"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["Illumina NovaSeq 6000"],"study_type":["RNA-seq of coding RNA"],"species":["Mus musculus"],"pubmed_authors":["Jianyao Xue"],"additional_accession":[]},"is_claimable":false,"name":"Hemin-induced DEGs in Lung, Kidney of WT and hepatic SR-BI KO mice","description":"Acute hemolysis can deplete haptoglobin and hemopexin, leaving toxic free heme insufficiently controlled. Here, the authors show that scavenger receptor BI (SR-BI) mediates hepatic uptake of free heme and is essential for protection against heme-induced injury and death. Here we examined gene expression post hemin injections","dates":{"release":"2026-07-24T00:00:00Z","modification":"2026-07-24T01:00:52.285Z","creation":"2026-07-15T14:23:46.188Z"},"accession":"E-MTAB-17338","cross_references":{"ENA":["ERP201272"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005518","EFO_0003816","EFO_0003738","EFO_0004184"]}}