biostudies-arrayexpressJianyao XueMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-17338Acute hemolysis can deplete haptoglobin and hemopexin, leaving toxic free heme insufficiently controlled. Here, the authors show that scavenger receptor BI (SR-BI) mediates hepatic uptake of free heme and is essential for protection against heme-induced injury and death. Here we examined gene expression post hemin injectionsbiostudies-arrayexpressSequencing - (1) Data quality control: filtering reads containing adapter or with low quality; (2) Statistics analysis of data production of quality; (3) Mapping reads to reference genome (STAR); (4) Gene expression quantification (HTseq); (5) Differential expression analysis (DEseq2/edgeR). pair-end reads were 150-base-pair ones, output is 9G. NovaSeq 6000 Illumina platform was usedNucleic Acid Extraction - Total RNA was isolated by using RNeasy mini kit from QiagenSample Collection - SR-BIfl/fl and AlbcreSR-BIfl/fl mice were treated with/without hemin at 40 µmol/kg BW, i.p. Twenty-four hours later, a second injection was performed. Thirty-eight hours post the initial injection, RNA was isolated from Kidney, lung and liver using Qiagen RNeasy Mini Kit, and sent to Novogene for RNA sequencing analysis.Library Construction - Total RNA was sent to Novogene for library constructionOrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - (1) Data quality control: filtering reads containing adapter or with low quality; (2) Statistics analysis of data production of quality; (3) Mapping reads to reference genome (STAR); (4) Gene expression quantification (HTseq); (5) Differential expression analysis (DEseq2/edgeR). pair-end reads were 150-base-pair ones, output is 9G. NovaSeq 6000 Illumina platform was used Supplementary files format and content: fpkm Supplementary files format and content: readcountMetabolomicsUnknownTranscriptomicsGenomicsProteomicsIllumina NovaSeq 6000RNA-seq of coding RNAMus musculusJianyao XuefalseHemin-induced DEGs in Lung, Kidney of WT and hepatic SR-BI KO miceAcute hemolysis can deplete haptoglobin and hemopexin, leaving toxic free heme insufficiently controlled. Here, the authors show that scavenger receptor BI (SR-BI) mediates hepatic uptake of free heme and is essential for protection against heme-induced injury and death. Here we examined gene expression post hemin injections2026-07-24T00:00:00Z2026-07-24T01:00:52.285Z2026-07-15T14:23:46.188ZE-MTAB-17338ERP201272EFO_0002944EFO_0004170EFO_0005518EFO_0003816EFO_0003738EFO_0004184