{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Angel Carlos Roman"],"organism":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-17353"],"description":["We aim to investigate the role of METTL3 and METTL14, the catalytic enzyme responsible for m⁶A methylation, in high-grade serous ovarian carcinoma (HGSOC) cells like OVCAR8 human cell line. In addition, we aimed to identify m⁶A-modified transcripts using MeRIP-seq in the same cell line."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Nucleic Acid Extraction - RNA was extracted using a standard RNA mini isolation kit.","Sample Collection - Cell cultures were maintained at 37ºC / 5% CO2 using standard DMEM media + 10% FBS","Sequencing - Illumina sequencing was done in Novogene, extracting 20M paired-end 75 bp reads","Library Construction - Libraries were built by Novogene using standard procedures for Illumina sequencing.","Nucleic Acid Extraction - MeRIP was used for immunoprecipitation of m6A-enriched of RNA"],"figure_sub":["Organization","MINSEQE Score","Assays and Data","Processed Data","MAGE-TAB Files"],"data_protocol":["Data Transformation - In the case of RNA-seq, Kallisto was used to pseudoalign and quantify transcript reads. In the case of MeRIP, MACS2 was used to detect peaks in a BWA-aligned data."],"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["Illumina NovaSeq 6000"],"study_type":["RNA-seq of coding RNA"],"species":["Homo sapiens"],"pubmed_authors":["Angel Carlos Roman"],"additional_accession":[]},"is_claimable":false,"name":"RNA-seq of human ovarian cancer cell line (OVCAR8) with modulation of METTL3 and METTL14","description":"We aim to investigate the role of METTL3 and METTL14, the catalytic enzyme responsible for m⁶A methylation, in high-grade serous ovarian carcinoma (HGSOC) cells like OVCAR8 human cell line. In addition, we aimed to identify m⁶A-modified transcripts using MeRIP-seq in the same cell line.","dates":{"release":"2026-09-14T00:00:00Z","modification":"2026-09-14T17:50:28.573Z","creation":"2026-08-31T20:07:51.087Z"},"accession":"E-MTAB-17353","cross_references":{"ENA":["ERP204731"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005518","EFO_0003816","EFO_0003738","EFO_0004184"]}}