biostudies-arrayexpressAngel Carlos RomanHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-17353We aim to investigate the role of METTL3 and METTL14, the catalytic enzyme responsible for m⁶A methylation, in high-grade serous ovarian carcinoma (HGSOC) cells like OVCAR8 human cell line. In addition, we aimed to identify m⁶A-modified transcripts using MeRIP-seq in the same cell line.biostudies-arrayexpressNucleic Acid Extraction - RNA was extracted using a standard RNA mini isolation kit.Sample Collection - Cell cultures were maintained at 37ºC / 5% CO2 using standard DMEM media + 10% FBSSequencing - Illumina sequencing was done in Novogene, extracting 20M paired-end 75 bp readsLibrary Construction - Libraries were built by Novogene using standard procedures for Illumina sequencing.Nucleic Acid Extraction - MeRIP was used for immunoprecipitation of m6A-enriched of RNAOrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - In the case of RNA-seq, Kallisto was used to pseudoalign and quantify transcript reads. In the case of MeRIP, MACS2 was used to detect peaks in a BWA-aligned data.MetabolomicsUnknownTranscriptomicsGenomicsProteomicsIllumina NovaSeq 6000RNA-seq of coding RNAHomo sapiensAngel Carlos RomanfalseRNA-seq of human ovarian cancer cell line (OVCAR8) with modulation of METTL3 and METTL14We aim to investigate the role of METTL3 and METTL14, the catalytic enzyme responsible for m⁶A methylation, in high-grade serous ovarian carcinoma (HGSOC) cells like OVCAR8 human cell line. In addition, we aimed to identify m⁶A-modified transcripts using MeRIP-seq in the same cell line.2026-09-14T00:00:00Z2026-09-14T17:50:28.573Z2026-08-31T20:07:51.087ZE-MTAB-17353ERP204731EFO_0002944EFO_0004170EFO_0005518EFO_0003816EFO_0003738EFO_0004184