biostudies-arrayexpressDag Marcus EideMus musculusnf-core/rnaseq pipeline v3.21.0 (Ewels et al., 2020) using Salmon against the GRCm39 mouse reference genome, summarised to gene level with tximeta packagehttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-17355Mouse testis and liver samples collected one day post-radiation/As-exposure liver and testis genome-wide transcriptional profiles in male mice of two strains (CBA/CaOlaHsd and C57BL/6NHsd) exposed chronically to a low dose rate (2.5 mGy/h; 1200h, LDR), mid-dose rate (10 mGy/h; 300h, MDR), acutely to a high dose rate (100 mGy/h; 30h, HDR) of gamma irradiation – all at total dose 3Gy, Co-60 source. Two additional groups were exposed acutely to 3Gy Xray with and without priming with LDR (i.e., 2 groups, X and LDR_X). Four additional groups were exposed to sodium arsenite (0.3 and 3 mg/L in drinking water, LAs and HAs, respectively), either arsenate alone (LAs, HAs) or combined with LDR gamma (Lg-LAs, Lg_HAs). 5 mice/group/strain standard, except 1 group of 3, 2 groups of 4. ONLY THE raw data for low dose rate gamma and arsenite exposed groups are currently uploaded. The analysis/publication of the testis data for the remaining groups is not yet finished, if ever. The liver raw data for the remaining groups have been deposited at PRJNA747753 (SVA),biostudies-arrayexpressLibrary Construction - RNA-Seq library-prep was performed using the TruSeq Stranded mRNA kit (v2, Illumina) (insert length 250~300) and paired-end sequencing (2 X 150 bp)Sequencing - prepared libraries for livers were sequenced on Illumina NovaSeq 6000 at Novogen Co., Ltd (Cambridge, UK) Testis samples were run on Illumina Novaseq Sp at the Norwegian sequencing centre (Oslo, Norway).Sample Collection - post mortem organ samples collected at termination snap frozen and stored at -80C until preparationNucleic Acid Extraction - Total RNA was isolated from liver tissue samples using the miRNeasy mini kit (Qiagen, Hilden, Germany, cat. #217004) according to the manufacturer's instructions. Lysis buffer (600 μl) were added to the frozen liver tissue and homogenized using stainless steel bead (5 mm) for 2×2 min (frequency: 20/s) using the TissueLyserII Homogenizer (Qiagen, Hilden, Germany)). Homogenized samples were spun at 10,000×g and the supernatants used for further RNA isolation. DNase digestion step was skipped out due to downstream poly-A mRNA enrichment. RNA quality and quantity were assessed using an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, California, USA) and a NanoDrop Spectrophotometer (Thermo Fisher Scientific, Massachusetts, USA).Sample Treatment - Gamma exposure: for 1200hrs (53 days) @ 2.5 mGy/h Co-60 collimator, or no irradiation - same room behind lead shield. Arsenite: 300 or 3000 microgram/L given as drinking water from day 7 until day 53, before that city tap water, which also was the control. Liver sample analysis ICP-MS confirmed large differences in [As] between the groups - no outliers or group-overlapping concentrations observed for any sampleOrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - raw counts were analysed with DEseq2 for DEGs or used for GSEA pathway analysis, vet normalised data used when transformed data were neededMetabolomicsUnknownTranscriptomicsGenomicsProteomicsIllumina NovaSeq 6000RNA-seq of total RNAMus musculusConcurrent low-dose-rate gamma irradiation and inorganic arsenite in mice: genotoxicity and transcriptional responses in liver and testis in CBA and C57BL/6 miceDag BredeDag M Eide, Anne Graupner, Birgitte Lindeman, Hildegunn Dahl, Dag A Brede, Nur Duale, Ann Karin OlsenDag Marcus EidefalseConcurrent low-dose-rate gamma irradiation and inorganic arsenite in mice: genotoxicity and transcriptional responses in liver and testis in CBA and C57BL/6 miceMouse testis and liver samples collected one day post-radiation/As-exposure liver and testis genome-wide transcriptional profiles in male mice of two strains (CBA/CaOlaHsd and C57BL/6NHsd) exposed chronically to a low dose rate (2.5 mGy/h; 1200h, LDR), mid-dose rate (10 mGy/h; 300h, MDR), acutely to a high dose rate (100 mGy/h; 30h, HDR) of gamma irradiation – all at total dose 3Gy, Co-60 source. Two additional groups were exposed acutely to 3Gy Xray with and without priming with LDR (i.e., 2 groups, X and LDR_X). Four additional groups were exposed to sodium arsenite (0.3 and 3 mg/L in drinking water, LAs and HAs, respectively), either arsenate alone (LAs, HAs) or combined with LDR gamma (Lg-LAs, Lg_HAs). 5 mice/group/strain standard, except 1 group of 3, 2 groups of 4. ONLY THE raw data for low dose rate gamma and arsenite exposed groups are currently uploaded. The analysis/publication of the testis data for the remaining groups is not yet finished, if ever. The liver raw data for the remaining groups have been deposited at PRJNA747753 (SVA),2026-08-28T00:00:00Z2026-08-28T01:00:50.629Z2026-07-17T16:53:54.169ZE-MTAB-17355ERP201409EFO_0002944EFO_0004170EFO_0009653EFO_0005518EFO_0003816EFO_0004184EFO_0003969