{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Julia Madeleine Heinze"],"organism":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-17384"],"description":["Mesenteric white adipose tissue from wildtype C57BL/6 and Wipf1-/- mice was harvested through vivosection, minced and digested. Tissue-resident lymphocytes were enriched with Percoll gradient centrifugation. CD45 staining and fluorescence associated cell sorting were performed to achieve a pure lymphocyte population. Single cell suspension and library preparation were performed with 20.000 of these cells on the 10x genomics platform and following the Chromium Next GEM Single Cell 3’ Reagent Kit User Guide Revision E. Illumina sequencing was performed."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sequencing - Illumina paired-end sequencing was performed by the Helmholtz Munich Genomics Core facility on a NovaSeq 6000","Nucleic Acid Extraction - Nucleic acid extraction was performed following the Chromium Next GEM Single Cell 3’ Reagent Kit User Guide Revision E.","Sample Collection - Mesenteric white adipose tissue from wildtype C57BL/6 and Wipf1-/- mice was harvested through vivosection, minced and digested. Tissue-resident lymphocytes were enriched with Percoll gradient centrifugation. CD45 staining and fluorescence associated cell sorting were performed to achieve a pure lymphocyte population.Single cell suspension was made with 20.000 of these cells on the 10x genomics platform and following the Chromium Next GEM Single Cell 3’ Reagent Kit User Guide Revision E.","Library Construction - Library preparation was performed following the Chromium Next GEM Single Cell 3’ Reagent Kit User Guide Revision E."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["BD Aria, 10X Chromium","Illumina NovaSeq 6000"],"study_type":["RNA-seq of coding RNA from single cells"],"species":["Mus musculus"],"pubmed_title":["B cell/adipocyte crosstalk drives pro-fibrotic macrophage migration and polarisation in IBD-associated inflamed white adipose tissue"],"pubmed_authors":["Marie Christine Goess 1,2 , Julia M. Heinze 1,2 , Julian Hofmann 3 , Annalisa Buck 4 , Iana Gadjalova 1,2 , Marie-Christin Weber 4 , Sandra Ring 1,2 , Matteo Villa 8 , Jörg Büscher 5 , Philipp- Alexander Neumann 3 , Jürgen Ruland 1,2,6,7 , Selina J. Keppler","Julia Madeleine Heinze","Selina Jessica Keppler"],"additional_accession":[]},"is_claimable":false,"name":"3' single cell sequencing of CD45+ lymphocytes sorted from mesenteric white adipose tissue in wildtype C57BL/6 and WIPf1-/- mice","description":"Mesenteric white adipose tissue from wildtype C57BL/6 and Wipf1-/- mice was harvested through vivosection, minced and digested. Tissue-resident lymphocytes were enriched with Percoll gradient centrifugation. CD45 staining and fluorescence associated cell sorting were performed to achieve a pure lymphocyte population. Single cell suspension and library preparation were performed with 20.000 of these cells on the 10x genomics platform and following the Chromium Next GEM Single Cell 3’ Reagent Kit User Guide Revision E. Illumina sequencing was performed.","dates":{"release":"2026-07-31T00:00:00Z","modification":"2026-07-31T13:26:48.315Z","creation":"2026-07-23T14:40:38.401Z"},"accession":"E-MTAB-17384","cross_references":{"ENA":["ERP202606"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005684","EFO_0005518","EFO_0004184"]}}