{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Neha Srivastava"],"organism":["Homo sapiens"],"software":["Not Applocable","Agilent Feature Extraction","Agilent Feature Extraction, Metabolist"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-17484"],"description":["Peripheral blood samples were collected from (Heavy metal exposer- Parkinson disease)HME-PD, (Heavy metal non-exposer- Parkinson disease) NHME-PD, and healthy controls. Total RNA was isolated and profiled using the Affymetrix GeneChip™ miRNA 3.1 Array. Arrays were hybridized, washed, stained, scanned, and raw data (.CEL files) were RMA-normalized for differential expression."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Scaning - The array scanning and feature extraction protocol for microRNA (miRNA) microarrays involves laser scanning the hybridized slide, aligning a grid template, and quantifying fluorescent intensities","Sample Collection - Based on clinical assessments, 2 ml of blood was drawn from each of the 10 PD patients in the heavy metal exposure group and 10 PD patients in the non- heavy metal exposure group, with 5 healthy controls from the heavy metal exposure group residing in the North East region. Blood was collected from the superficial vein on the upper limb and subsequently frozen at -20°C for subsequent analysis.","Labeling - The research was conducted using Affymetrix miRNA 3.1, which contains probes for human mature and pre-miRNA hairpins. After creating and reverse transcribing antisense cRNA, dUTP was incorporated into the cDNA. The cDNA was broken up using apurinic/apyrimidinic endonuclease I and uracil DNA glycosylase.","Nucleic Acid Extraction - Using the TRIzol reagent (Invitrogen, Carlsbad, CA, USA), total RNA was extracted from the whole blood samples in each group with respective controls. A 2% agarose gel has been used to confirm the integrity of the RNA.  Following that, the RNA sample was kept at -80 for further experiments.","Hybridization - Affymetrix miRNA3.1 arrays were hybridized for 20 hours at 55ºC and 20 rpm of continuous speed rotation. Using fluorescently-labeled antibodies, the miRNA array was cleaned, collared, and scanned once more in search of hybridization signals."],"figure_sub":["MIAME Score","Organization","Assays and Data","Processed Data","MAGE-TAB Files","Array Designs"],"data_protocol":["Data Transformation - Normalized miRNA expression data were analyzed using the Metaboanalyst 6.0 (https://www.metaboanalyst.ca/MetaboAnalyst/ModuleView.xhtml), a comprehensive web-based platform used for metabolomics data analysis, visualization, and interpretation, and validated"],"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["Not Applicablre","Not Applicable","Affymetrix miRNA 3.1"],"study_type":["microRNA profiling by array"],"species":["Homo sapiens"],"pubmed_authors":["Neha Srivastava"],"additional_accession":[]},"is_claimable":false,"name":"MiRNA expression profiling in Parkinson disease patients from Heavy metal exposer and non exposer group with respective control","description":"Peripheral blood samples were collected from (Heavy metal exposer- Parkinson disease)HME-PD, (Heavy metal non-exposer- Parkinson disease) NHME-PD, and healthy controls. Total RNA was isolated and profiled using the Affymetrix GeneChip™ miRNA 3.1 Array. Arrays were hybridized, washed, stained, scanned, and raw data (.CEL files) were RMA-normalized for differential expression.","dates":{"release":"2026-08-31T00:00:00Z","modification":"2026-08-31T01:00:47.408Z","creation":"2026-08-14T12:35:16.615Z"},"accession":"E-MTAB-17484","cross_references":{"EFO":["EFO_0002944","EFO_0003814","EFO_0000753","EFO_0003813","EFO_0005518","EFO_0003816","EFO_0003815"]}}