biostudies-arrayexpressNeha SrivastavaHomo sapiensNot ApplocableAgilent Feature ExtractionAgilent Feature Extraction, Metabolisthttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-17484Peripheral blood samples were collected from (Heavy metal exposer- Parkinson disease)HME-PD, (Heavy metal non-exposer- Parkinson disease) NHME-PD, and healthy controls. Total RNA was isolated and profiled using the Affymetrix GeneChip™ miRNA 3.1 Array. Arrays were hybridized, washed, stained, scanned, and raw data (.CEL files) were RMA-normalized for differential expression.biostudies-arrayexpressScaning - The array scanning and feature extraction protocol for microRNA (miRNA) microarrays involves laser scanning the hybridized slide, aligning a grid template, and quantifying fluorescent intensitiesSample Collection - Based on clinical assessments, 2 ml of blood was drawn from each of the 10 PD patients in the heavy metal exposure group and 10 PD patients in the non- heavy metal exposure group, with 5 healthy controls from the heavy metal exposure group residing in the North East region. Blood was collected from the superficial vein on the upper limb and subsequently frozen at -20°C for subsequent analysis.Labeling - The research was conducted using Affymetrix miRNA 3.1, which contains probes for human mature and pre-miRNA hairpins. After creating and reverse transcribing antisense cRNA, dUTP was incorporated into the cDNA. The cDNA was broken up using apurinic/apyrimidinic endonuclease I and uracil DNA glycosylase.Nucleic Acid Extraction - Using the TRIzol reagent (Invitrogen, Carlsbad, CA, USA), total RNA was extracted from the whole blood samples in each group with respective controls. A 2% agarose gel has been used to confirm the integrity of the RNA. Following that, the RNA sample was kept at -80 for further experiments.Hybridization - Affymetrix miRNA3.1 arrays were hybridized for 20 hours at 55ºC and 20 rpm of continuous speed rotation. Using fluorescently-labeled antibodies, the miRNA array was cleaned, collared, and scanned once more in search of hybridization signals.MIAME ScoreOrganizationAssays and DataProcessed DataMAGE-TAB FilesArray DesignsData Transformation - Normalized miRNA expression data were analyzed using the Metaboanalyst 6.0 (https://www.metaboanalyst.ca/MetaboAnalyst/ModuleView.xhtml), a comprehensive web-based platform used for metabolomics data analysis, visualization, and interpretation, and validatedMetabolomicsUnknownTranscriptomicsGenomicsProteomicsNot ApplicablreNot ApplicableAffymetrix miRNA 3.1microRNA profiling by arrayHomo sapiensNeha SrivastavafalseMiRNA expression profiling in Parkinson disease patients from Heavy metal exposer and non exposer group with respective controlPeripheral blood samples were collected from (Heavy metal exposer- Parkinson disease)HME-PD, (Heavy metal non-exposer- Parkinson disease) NHME-PD, and healthy controls. Total RNA was isolated and profiled using the Affymetrix GeneChip™ miRNA 3.1 Array. Arrays were hybridized, washed, stained, scanned, and raw data (.CEL files) were RMA-normalized for differential expression.2026-08-31T00:00:00Z2026-08-31T01:00:47.408Z2026-08-14T12:35:16.615ZE-MTAB-17484EFO_0002944EFO_0003814EFO_0000753EFO_0003813EFO_0005518EFO_0003816EFO_0003815