{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Andreas Bachmair"],"organism":["Arabidopsis thaliana"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-17503"],"description":["SUMO conjugation is an important process in developmental transitions and in environmental stress responses. We generated mutants in the key enzyme SUMO conjugating enzyme 1 of Arabidopsis thaliana and investigated how these mutations affect gene expression in leaves. We also combined mutations in SUMO conjugation with expression of a bacterial nahG gene that destroys salicylic acid, to determine which of the differently regulated genes depend on increased salicylic acid levels. The experiment encompasses four biological replicates for each genotype."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sample Collection - Leaves were collected from rosettes of plants with first flowers opening. Leaves were frozen in liquid nitrogen.","Nucleic Acid Extraction - Frozen leaf material was crushed with metal beads in a Qiagen TissueLyseer II and used to extract RNA with Promega SV Total RNA Isolation System acc. to manufacturer instructions.","Growth Protocol - Plants were grown in a controlled environment climate chamber at 22°C 12/8 day/night cycle at 150 µE light form an LED panel.","Sequencing - Sequencing was carried with a NovaSeqX sequencer with 1400M reads.","Library Construction - PolyA RNA was enriched using the NEBNext Poly(A) mRNA Magnetic Isolation Module followed by library construction with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","Processed Data","MAGE-TAB Files"],"data_protocol":["Data Transformation - DEseq2_1.18 was used for normalisation, values at alpha0.05 wer designated as significantly \\\"up\\\" or \"down\\"],"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["Illumina NovaSeq X"],"study_type":["RNA-seq of coding RNA"],"species":["Arabidopsis thaliana"],"pubmed_authors":["Andreas Bachmair"],"additional_accession":[]},"is_claimable":false,"name":"Comparative RNA expression analysis of Arabidopsis leaf material differing in genes of SUMO conjugation","description":"SUMO conjugation is an important process in developmental transitions and in environmental stress responses. We generated mutants in the key enzyme SUMO conjugating enzyme 1 of Arabidopsis thaliana and investigated how these mutations affect gene expression in leaves. We also combined mutations in SUMO conjugation with expression of a bacterial nahG gene that destroys salicylic acid, to determine which of the differently regulated genes depend on increased salicylic acid levels. The experiment encompasses four biological replicates for each genotype.","dates":{"release":"2026-09-01T00:00:00Z","modification":"2026-09-01T01:00:53.008Z","creation":"2026-08-13T20:17:41.566Z"},"accession":"E-MTAB-17503","cross_references":{"ENA":["ERP203684"],"EFO":["EFO_0002944","EFO_0004170","EFO_0003789","EFO_0005518","EFO_0003816","EFO_0003738","EFO_0004184"]}}