biostudies-arrayexpressAndreas BachmairArabidopsis thalianahttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-17503SUMO conjugation is an important process in developmental transitions and in environmental stress responses. We generated mutants in the key enzyme SUMO conjugating enzyme 1 of Arabidopsis thaliana and investigated how these mutations affect gene expression in leaves. We also combined mutations in SUMO conjugation with expression of a bacterial nahG gene that destroys salicylic acid, to determine which of the differently regulated genes depend on increased salicylic acid levels. The experiment encompasses four biological replicates for each genotype.biostudies-arrayexpressSample Collection - Leaves were collected from rosettes of plants with first flowers opening. Leaves were frozen in liquid nitrogen.Nucleic Acid Extraction - Frozen leaf material was crushed with metal beads in a Qiagen TissueLyseer II and used to extract RNA with Promega SV Total RNA Isolation System acc. to manufacturer instructions.Growth Protocol - Plants were grown in a controlled environment climate chamber at 22°C 12/8 day/night cycle at 150 µE light form an LED panel.Sequencing - Sequencing was carried with a NovaSeqX sequencer with 1400M reads.Library Construction - PolyA RNA was enriched using the NEBNext Poly(A) mRNA Magnetic Isolation Module followed by library construction with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina.OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - DEseq2_1.18 was used for normalisation, values at alpha0.05 wer designated as significantly \"up\" or "down\MetabolomicsUnknownTranscriptomicsGenomicsProteomicsIllumina NovaSeq XRNA-seq of coding RNAArabidopsis thalianaAndreas BachmairfalseComparative RNA expression analysis of Arabidopsis leaf material differing in genes of SUMO conjugationSUMO conjugation is an important process in developmental transitions and in environmental stress responses. We generated mutants in the key enzyme SUMO conjugating enzyme 1 of Arabidopsis thaliana and investigated how these mutations affect gene expression in leaves. We also combined mutations in SUMO conjugation with expression of a bacterial nahG gene that destroys salicylic acid, to determine which of the differently regulated genes depend on increased salicylic acid levels. The experiment encompasses four biological replicates for each genotype.2026-09-01T00:00:00Z2026-09-01T01:00:53.008Z2026-08-13T20:17:41.566ZE-MTAB-17503ERP203684EFO_0002944EFO_0004170EFO_0003789EFO_0005518EFO_0003816EFO_0003738EFO_0004184