{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Elaine Emmerson"],"instrument_platform":["Illumina HiSeq 2500"],"study_type":["RNA-seq of coding RNA"],"organism":["Mus musculus"],"species":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-17509"],"description":["The salivary glands often become damaged in individuals receiving radiotherapy for head and neck cancer, resulting in chronic dry mouth. This leads to detrimental effects on their health and quality of life, for which there is no regenerative therapy. Progenitor cells can replenish damaged tissue. We have shown the macrophages interact with KRT14+ basal epithelial progenitor cells; however, their function is unknown. Here, using bulk RNAseq we profile the transcriptomes of KRT14+ cells isolated from adult mouse submandibular salivary gland 14 days after targeted irradiation which is either macrophage-proficient or macrophage-deficient. For each sample equal numbers of cells from 8 mice were pooled."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Library Construction - RNA fragmentation, first- and second-strand cDNA synthesis (reverse transcription), adapter ligation, and library amplification","Sample Collection - Salivary gland tissue was excised, enzymatically and mechanically digested and Epcam+Krt14+ cells (epithelial cells), CD31+ cells (endothelial cells) and CD45+/CD11b+/F480+ cells (macrophages) were isolated using fluorescence-activated cell sorting (FACS).","Nucleic Acid Extraction - Nucleic acid was extracted using the Genewiz Ultra-low input protocol.","Sequencing - Sequencing was performed on two lanes on a HiSeq Sequencing System (Illumina) with the following cycle setup for paired-end reads: read 1, 28 cycles; i7 index, 8 cycles; read 2, 91 cycles."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Elaine Emmerson"],"additional_accession":[]},"is_claimable":false,"name":"RNAseq of KRT14+ progenitor cells from macrophage-proficient versus macrophage deficient mouse submandibular salivary gland","description":"The salivary glands often become damaged in individuals receiving radiotherapy for head and neck cancer, resulting in chronic dry mouth. This leads to detrimental effects on their health and quality of life, for which there is no regenerative therapy. Progenitor cells can replenish damaged tissue. We have shown the macrophages interact with KRT14+ basal epithelial progenitor cells; however, their function is unknown. Here, using bulk RNAseq we profile the transcriptomes of KRT14+ cells isolated from adult mouse submandibular salivary gland 14 days after targeted irradiation which is either macrophage-proficient or macrophage-deficient. For each sample equal numbers of cells from 8 mice were pooled.","dates":{"release":"2026-08-10T00:00:00Z","modification":"2026-08-10T01:00:43.143Z","creation":"2026-08-07T14:49:00.592Z"},"accession":"E-MTAB-17509","cross_references":{"ENA":["ERP203500"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005518","EFO_0003738","EFO_0004184"]}}