biostudies-arrayexpressMassimo ZolloHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-17520Caco-2 cells were plated (1x106 cells) and treated with a nutraceutical formula (Solution-10) at 0.01x concentration or with vehicle (saline solution) as the negative control for the treatment. After 24 hours, the cells were lysed, and their RNA was extracted for RNAseq.biostudies-arrayexpressSequencing - Type of sequencer: Illumina platform. Sequencing protocol: NovaSeq 6000 System User Guide Document # 1000000019358 v02; Sequencing Control Software: 1000000019358 v02; Trimmed reads are mapped to reference genome with HISAT2 (https://ccb.jhu.edu/software/hisat2/index.shtml), splice-aware aligner. After the read mapping, Stringtie (https://ccb.jhu.edu/software/stringtie/) was used for transcript assembly.Nucleic Acid Extraction - Total RNA, isolated from Caco-2 cells by using TRIzol RNA Isolation Reagent (#15596018; Ambion, Thermo Fisher Scientific) was quantified in a NanoDrop™ One/OneC Microvolume UV-Vis Spectrophotometer (Thermo Scientific™), checked for purity and integrity.Growth Protocol - Caco-2 (ATCC, Middlesex, UK; accession number: HTB-37 L) cells were grown in a humidified 37°C incubator with 5% CO2. The cells were cultured in feeder-free conditions using Dulbecco’s modified Eagle’s medium (41966-029; Gibco) with 10% fetal bovine serum (10270-106; Gibco), 2 mM L-glutamine (25030-024; Gibco), and 1% penicil-lin/streptomycin (P0781; Sigma-Aldrich), with medium changed daily. Cells were dis-sociated with Trypsin-EDTA solution (T4049, Sigma-Aldrich) when the culture reached ~80% confluency.Library Construction - Libraries were prepared using the TruSeq® Stranded mRNA Library Kit according to the protocols recommended by the manufacturer. Library kit: TruSeq® Stranded mRNASample Treatment - Caco-2 cells (ATCC, Middlesex, UK; accession number: HTB-37 L) were plated (1x106 cells) and treated for 24 hours with a nutraceutical formula (Solution-10) at 0.01x concentration or with vehicle (saline solution) as the negative control for the treatment.Sample Collection - Caco-2 cells (ATCC, Middlesex, UK; accession number: HTB-37 L) were plated (1x106 cells) and treated for 24 hours with a nutraceutical formula (Solution-10) at 0.01x concentration or with vehicle (saline solution) as the negative control for the treatment. After 24 hours, the cells were lysed with TRIzol RNA Isolation Reagent (0.5 ml, #15596018; Ambion, Thermo Fisher Scientific) and frozen at -80°C.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesData Transformation - Trimmed reads are mapped to reference genome with HISAT2, splice-aware aligner. Transcript is assembled by StringTie with aligned reads. Expression profiles are represented as read count and normalization value which is based on transcript length and depth of coverage. The FPKM (Fragments Per Kilobase of transcript per Million Mapped reads) value is used as a normalization value.Sequence Alignment - Trimmed reads are mapped to reference genome with HISAT2 (https://ccb.jhu.edu/software/hisat2/index.shtml), splice-aware aligner. After the read mapping, Stringtie (https://ccb.jhu.edu/software/stringtie/) was used for transcript assembly.UnknownTranscriptomicsGenomicsProteomicsIllumina NovaSeq 6000RNA-seq of coding RNAHomo sapiensTranscriptomic reprogramming by an epithelial immune-modulating formulation linking gut–brain communication, inflammaging, neurodegeneration and longevityVeronica Ferrucci1,2 #, Francesca Zollo2,3, Martina Castellano2, Luigi Scarpato4, Nicola Capuano5, Vincenzo Diego Bianchi6 and Massimo Zollo1,2,7# 1 Department of Molecular Medicine and Medical Biotechnologies, DMMBM, University of Naples ‘Federico II’, Via Pansini 5, 80131, Naples, Italy. 2 Central Nervous System laboratory (CNS), CEINGE-Biotecnologie Avanzate “Franco Salvatore” s.c.a.r.l., Via Gaetano Salvatore 486, 80145, Naples, Italy. 3 Dipartimento di Scienze Biochimiche A. Rossi Fanelli, Dottorato di Biologia Computazionale e Bioinformatica, University of Rome \"La Sapienza\", Rome, Italy. 4 Department of Melanoma, Cancer Immunotherapy and Development Therapeutics, Istituto Nazionale Tumori IRCCS \"Fondazione G. Pascale\", 80131 Naples, Italy. 5 Department of Orthopaedic and TraumaMassimo ZollofalseRNA-seq of human Caco-2 cells treated with a nutraceutical formula (solution-10), or vehicle-(saline solution)- controlCaco-2 cells were plated (1x106 cells) and treated with a nutraceutical formula (Solution-10) at 0.01x concentration or with vehicle (saline solution) as the negative control for the treatment. After 24 hours, the cells were lysed, and their RNA was extracted for RNAseq.2026-08-14T00:00:00Z2026-08-14T01:18:45.319Z2026-08-14T01:18:29.63ZE-MTAB-17520ERP203693EFO_0002944EFO_0004170EFO_0003789EFO_0004917EFO_0005518EFO_0003816EFO_0003738EFO_0004184EFO_0003969