biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsKai FangDNBelab C‑TaiM 4 droplet generator (MGI, China); DNBSEQ‑T7 sequencing platform (MGI, China).Illumina NovaSeq 6000RNA-seq of total RNA from single cellsHomo sapiensHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-17535Single‑cell RNA sequencing was performed on 10 pairs of tumor tissues and matched adjacent normal tissues from gastric cancer patients.biostudies-arrayexpressSequencing - Sequencing was performed on the DNBSEQ‑T7 sequencing platform (MGI, China) using the paired‑end 100 bp (PE100) strategy. Raw sequencing data were generated for subsequent single‑cell RNA‑seq data analysis.Nucleic Acid Extraction - Single‑cell suspensions were directly subjected to library construction using the DNBelab C Series kit, and no independent nucleic‑acid extraction step was performed prior to droplet encapsulation.Library Construction - Single‑cell suspensions were directly subjected to library construction using the DNBelab C Series High‑throughput Single‑cell RNA Library Preparation Kit V3.0 (MGI, China). No independent nucleic acid extraction step was performed prior to droplet encapsulation on the DNBelab C‑TaiM 4 droplet generator. Library quality was validated before sequencing on the DNBSEQ‑T7 platform with the PE100 strategy.Sample Collection - Patients with newly diagnosed gastric cancer who had received no prior anti‑tumor therapy and had no other malignancies were enrolled. Tissue specimens were surgically resected during surgery for single‑cell RNA‑seq analysis.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesKai FangfalseGastric cancerSingle‑cell RNA sequencing was performed on 10 pairs of tumor tissues and matched adjacent normal tissues from gastric cancer patients.2026-09-07T00:00:00Z2026-09-07T01:00:43.078Z2026-08-25T20:25:22.38ZE-MTAB-17535ERP204250EFO_0002944EFO_0004170EFO_0005518EFO_0004184