{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Dave Boucher"],"organism":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-17572"],"description":["Human NK cells (2 donors, healthy)   Up: Unprimed IL12: Primed with IL12 Sal: Salmonella SL1344 (log) infected cells) VX: VX765, inflammatory caspase inhibitor"],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sample Collection - Human NK cells was isolated by negative selection from healthy human blood. When indicated, cells were treated with IL12 overnight (100 ng/mL).  Sample were treated and collected after 5 hrs of infection with Salmonella (MOI 50).  When indicated, cells are treated with VX765 (25 micromolar) , 1 hr before infection, to imhibit inflammasome. 500k cells per condition were harvested by centrifugation and resuspended in Zymo buffer","Nucleic Acid Extraction - Zymo DNA/RNA Shield was used to resuspend the cell pellet. RNA was purified and processed by Plasmidsaurus","Sequencing - Plasmidsaurus standard procedure, and were sequenced according to manufacturer procedures","Library Construction - Plasmidsaurus standard procedure, and were sequenced according to manufacturer procedures"],"figure_sub":["Organization","MINSEQE Score","Assays and Data","Processed Data","MAGE-TAB Files"],"data_protocol":["Data Transformation - Data was normalised on the total read count (normalisation on CPM)"],"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["Illumina NovaSeq X"],"study_type":["RNA-seq of coding RNA"],"species":["Homo sapiens"],"pubmed_title":["Human NK cells engage cell-intrinsic non-canonical inflammasome function"],"pubmed_authors":["Nathan Leborgne","Leborgne et al","Dave Boucher"],"additional_accession":[]},"is_claimable":false,"name":"Effect of Salmonella infection and IL12 on human Natural Killer cells","description":"Human NK cells (2 donors, healthy)   Up: Unprimed IL12: Primed with IL12 Sal: Salmonella SL1344 (log) infected cells) VX: VX765, inflammatory caspase inhibitor","dates":{"release":"2026-09-14T00:00:00Z","modification":"2026-09-17T10:39:17.405Z","creation":"2026-09-04T11:40:37.504Z"},"accession":"E-MTAB-17572","cross_references":{"ENA":["ERP204945"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005518","EFO_0003816","EFO_0003738","EFO_0004184"]}}