biostudies-arrayexpressDave BoucherHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-17572Human NK cells (2 donors, healthy) Up: Unprimed IL12: Primed with IL12 Sal: Salmonella SL1344 (log) infected cells) VX: VX765, inflammatory caspase inhibitorbiostudies-arrayexpressSample Collection - Human NK cells was isolated by negative selection from healthy human blood. When indicated, cells were treated with IL12 overnight (100 ng/mL). Sample were treated and collected after 5 hrs of infection with Salmonella (MOI 50). When indicated, cells are treated with VX765 (25 micromolar) , 1 hr before infection, to imhibit inflammasome. 500k cells per condition were harvested by centrifugation and resuspended in Zymo bufferNucleic Acid Extraction - Zymo DNA/RNA Shield was used to resuspend the cell pellet. RNA was purified and processed by PlasmidsaurusSequencing - Plasmidsaurus standard procedure, and were sequenced according to manufacturer proceduresLibrary Construction - Plasmidsaurus standard procedure, and were sequenced according to manufacturer proceduresOrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - Data was normalised on the total read count (normalisation on CPM)MetabolomicsUnknownTranscriptomicsGenomicsProteomicsIllumina NovaSeq XRNA-seq of coding RNAHomo sapiensHuman NK cells engage cell-intrinsic non-canonical inflammasome functionNathan LeborgneLeborgne et alDave BoucherfalseEffect of Salmonella infection and IL12 on human Natural Killer cellsHuman NK cells (2 donors, healthy) Up: Unprimed IL12: Primed with IL12 Sal: Salmonella SL1344 (log) infected cells) VX: VX765, inflammatory caspase inhibitor2026-09-14T00:00:00Z2026-09-17T10:39:17.405Z2026-09-04T11:40:37.504ZE-MTAB-17572ERP204945EFO_0002944EFO_0004170EFO_0005518EFO_0003816EFO_0003738EFO_0004184