{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Juhi V Sur"],"study_type":["transcription profiling by array"],"organism":["Mus musculus"],"species":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-17587"],"description":["We investigated the impact of smooth-muscle–specific deletion of titin M-band exons 1–2. Understanding how disruption of titin M-band signaling alters uterine smooth-muscle physiology provides insight into the molecular mechanisms controlling coordinated uterine peristalsis, embryo positioning, and implantation. Uterine tissue was collected from E3.5 pregnant conditional titin M-band–deficient and control mice, and RNA was isolated for gene expression profiling using Illumina MouseRef-8 v2.0 Expression BeadChips. Expression data were quantile normalized in GenomeStudio and used for comparative transcriptomic analysis."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Hybridization - Gene expression profiling was performed using Illumina MouseRef-8 v2.0 Expression BeadChips. Biotinylated cRNA was hybridized to the arrays according to the manufacturer's instructions.","Nucleic Acid Extraction - Total RNA from left ventricular tissue was isolated using TRIzol (Thermofisher), treated with DNAseI (Qiagen), followed by a clean-up with the RNA micro kit (Qiagen). RNA quality was assessed using an Agilent 2100 Bioanalyzer prior to downstream processing.Ribosomal RNA (rRNA) was reduced using the RiboMinus Kit (Invitrogen) according to the manufacturer's instructions.","Sample Collection - Uterus tissue with embryo were collected from young adult (100 days old). After cervical dislocation, Uterus were excised, whole uterine tissue was  snap frozen and stored at -80°C until further processing. Tissue was cryopulverized with a mortar and pestle under liquid nitrogen.","Scaning - Illumina MouseRef-8 v2.0 Expression BeadChips were processed and scanned according to the manufacturer's instructions.","Labeling - Approximately 100 ng of total RNA was used as starting material. Biotinylated cRNA was generated using the Illumina TotalPrep-96 RNA Amplification Kit (Life Technologies) according to the manufacturer's instructions."],"figure_sub":["MIAME Score","Raw Data","Organization","Assays and Data","MAGE-TAB Files","Array Designs"],"pubmed_authors":["Juhi V Sur","Michael Gotthardt"],"data_protocol":["Data Transformation - Microarray expression data were processed using GenomeStudio V2011.1 with the Gene Expression Module V1.9.0. Expression values were quantile normalized. Only genes with expression levels significantly above background (detection p-value < 0.05) were considered for downstream analysis."],"additional_accession":[]},"is_claimable":false,"name":"The smooth muscle titin filament system regulates uterus contractility and implantation.","description":"We investigated the impact of smooth-muscle–specific deletion of titin M-band exons 1–2. Understanding how disruption of titin M-band signaling alters uterine smooth-muscle physiology provides insight into the molecular mechanisms controlling coordinated uterine peristalsis, embryo positioning, and implantation. Uterine tissue was collected from E3.5 pregnant conditional titin M-band–deficient and control mice, and RNA was isolated for gene expression profiling using Illumina MouseRef-8 v2.0 Expression BeadChips. Expression data were quantile normalized in GenomeStudio and used for comparative transcriptomic analysis.","dates":{"release":"2026-09-16T00:00:00Z","modification":"2026-09-17T10:39:36.141Z","creation":"2026-08-27T10:57:22.241Z"},"accession":"E-MTAB-17587","cross_references":{"EFO":["EFO_0002768","EFO_0002944","EFO_0003814","EFO_0003813","EFO_0005518","EFO_0003816","EFO_0003815"]}}