biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsJooa MoonIllumina NovaSeq 6000IlluminaRNA-seq of coding RNALupinus angustifoliusLupinus angustifoliushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-17708Total RNA was isolated from 100 mg of powdered tissue using the SpectrumTM Plant Total RNA Kit (Sigma-Aldrich), which included on-column DNAse treatment to remove genomic DNA contamination. The purified RNA was flash-frozen and dispatched to Macrogen (Seoul, Korea) for library preparation and sequencing. Following quality control checks, sequencing libraries were prepared using the TruSeq Stranded mRNA LT Sample Prep Kit (Illumina) as per the manufacturer's protocol (Part #15031047 Rev. E). The libraries were sequenced on an Illumina platform, producing 151-bp paired-end reads, with each sample yielding between 64–87 million paired-end reads.biostudies-arrayexpressNucleic Acid Extraction - Total RNA was isolated from 100 mg of powdered tissue using the SpectrumTM Plant Total RNA Kit (Sigma-Aldrich), which included on-column DNAse treatment to remove genomic DNA contamination.Sequencing - The libraries were sequenced on an Illumina platform, producing 151-bp paired-end reads, with each sample yielding between 64–87 million paired-end reads.Library Construction - The purified RNA was flash-frozen and dispatched to Macrogen (Seoul, Korea) for library preparation and sequencing. Following quality control checks, sequencing libraries were prepared using the TruSeq Stranded mRNA LT Sample Prep Kit (Illumina) as per the manufacturer's protocol (Part #15031047 Rev. E).Sample Collection - Leaves from 4-week-old plants were collected between 1-2 pm, flash-frozen in liquid nitrogen, and stored at -70°C prior to homogenization with a mortar and pestle cooled by liquid nitrogen.Growth Protocol - WT: Routine plant growth took place in a growth chamber at a light regime of 16h/8h (light/dark), temperatures of 20/18°C (day/night), relative humidity of 60%, and light intensity of 220 µmol m-2s-1. We used pots of 20 cm of depth and 16 cm of width containing a commercial potting mix. mutant:Seeds were sown in 40-cell trays without inoculation with rhizobia. 3-4-week-old seedlings were potted individually in 11,3 × 21,5-cm pots or in pairs in 19,5 × 25,5-cm pots (diameter/height). The pots were filled with a commercial peat-based substrate. The plants were grown under 18 hours of light, day/night temperatures of around 20/17°C, and a relative humidity between 30-60%. Fertilization was applied weekly with a liquid complete fertilizer 0,25% (NPK 18-11-18).OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesJooa MoonfalseRNA-seq of NLL cv. Oskar from 12 in-house leaf and stem control and RAP 2-7 mutant samplesTotal RNA was isolated from 100 mg of powdered tissue using the SpectrumTM Plant Total RNA Kit (Sigma-Aldrich), which included on-column DNAse treatment to remove genomic DNA contamination. The purified RNA was flash-frozen and dispatched to Macrogen (Seoul, Korea) for library preparation and sequencing. Following quality control checks, sequencing libraries were prepared using the TruSeq Stranded mRNA LT Sample Prep Kit (Illumina) as per the manufacturer's protocol (Part #15031047 Rev. E). The libraries were sequenced on an Illumina platform, producing 151-bp paired-end reads, with each sample yielding between 64–87 million paired-end reads.2026-10-05T00:00:00Z2026-10-05T07:48:18.917Z2026-10-01T15:04:23.432ZE-MTAB-17708ERP207287EFO_0002944EFO_0004170EFO_0003789EFO_0005518EFO_0003738EFO_0004184