biostudies-arrayexpressPavana ThomasHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-17726ROCK2 is a protein shown to be involved in radiation resistance. This study performed live sorting of SiHa cells (a cervical cancer cell line) on the basis of ROCK2 expression. R2High are cells with increased ROCK2 expression and R2Low are cells with decreased ROCK2 expression.biostudies-arrayexpressSample Collection - Blood was collected in EDTA vacutainers, buffy coat was collected and RBC lysis was performed.Sequencing - RNA was sequenced using the Illumina HiSeq paired-end sequencing (150 × 2). The raw data generated was checked for quality using FastQC and pre-processed, which includes removing the adapter sequences and the low-quality bases. Pre-processing of the data was done with the ABLT proprietary script. The reference genome used was the Homo sapiens DRCh38 build genome downloaded from the Ensemble database. Tophat-2.0.13 aligned the transcript sequences, and Cufflinks-2.2.1 performed assembly and differential expression. Cuffmerge created a combined assembly, while the Cuffdiff package performed Differential Gene Expression (DGE) analysis.Nucleic Acid Extraction - The RNA was isolated by the TRIzol method as described by the manufaacturer. Quantification was performed using the Qubit Fluorometer.Library Construction - The NEBNext Ultra Directional RNA Library Prep kit (E7420L) was utilized for RNA extraction and RNA-seq library preparation.OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - FPKM was used for normalization of data for within-sample comparison.MetabolomicsUnknownTranscriptomicsGenomicsProteomicsIllumina HiSeq 1000RNA-seq of coding RNAHomo sapiensPavana ThomasfalseRNA-seq of ROCK2 live-sorted cellsROCK2 is a protein shown to be involved in radiation resistance. This study performed live sorting of SiHa cells (a cervical cancer cell line) on the basis of ROCK2 expression. R2High are cells with increased ROCK2 expression and R2Low are cells with decreased ROCK2 expression.2026-09-30T00:00:00Z2026-09-30T01:01:14.361Z2026-09-29T08:34:11.801ZE-MTAB-17726ERP206969EFO_0002944EFO_0004170EFO_0005518EFO_0003816EFO_0003738EFO_0004184