{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Rui Martinho"],"organism":["Drosophila melanogaster"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-2321"],"description":["Transcriptome sequencing of a D. melanogaster mutant (fandango) that affects splicing, particularly in Early Zygotic genes"],"repository":["biostudies-arrayexpress"],"sample_protocol":["Nucleic Acid Extraction - Total RNA was isolated from 0-3Hs collections of fandango maternal mutant and control (FRT42B) embryos using TRIzol Reagent (Invitrogen) following standard protocol. To the RNA samples was performed a DNAse I (Promega) treatment during 30 min at 37C. After, the DNAse was extracted by Phenol-Chloroform extraction, the RNA was pellet by ethanol precipitation and dissolved it in 25 ul of DEPC water. We performed a Bioanalyzer test to analyze the quality and concentration of the samples and finally we brought the volume to 100 ul with water, added 50 ul of 7.5 M NH4OAc, 0.5 ul of glycogen and 250 ul of absolute ethanol.","Growth Protocol - Flies were raised using standard techniques. The fandango alleles were isolated from a previously reported maternal screen (Pimenta-Marques et al., 2008).","Sample Treatment - Flies were raised using standard techniques.","Sequencing - cDNA library was sequenced using the standard Illumina protocols","Library Construction - cDNA library was generated using the standard Illumina protocol for RNA-seq (polyA RNAs)."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["Illumina HiSeq 2000"],"study_type":["RNA-seq of coding RNA"],"species":["Drosophila melanogaster"],"pubmed_title":["Requirement for highly efficient pre-mRNA splicing during Drosophila early embryonic development"],"pubmed_authors":["Rui Martinho","Leonardo Guilgur, Pedro Prudêncio, Daniel Sobral, Denisa Liszekova, André Rosa, Rui Martinho","Daniel Sobral"],"additional_accession":[]},"is_claimable":false,"name":"RNA-seq of coding RNA of fandango, a Drosophila melanogaster mutant affecting splicing","description":"Transcriptome sequencing of a D. melanogaster mutant (fandango) that affects splicing, particularly in Early Zygotic genes","dates":{"release":"2014-03-25T00:00:00Z","modification":"2022-01-26T00:10:39.474Z","creation":"2022-01-26T00:10:39.474Z"},"accession":"E-MTAB-2321","cross_references":{"ENA":["ERP004884"],"EFO":["EFO_0003738"]}}