biostudies-arrayexpressRui MartinhoDrosophila melanogasterhttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-2321Transcriptome sequencing of a D. melanogaster mutant (fandango) that affects splicing, particularly in Early Zygotic genesbiostudies-arrayexpressNucleic Acid Extraction - Total RNA was isolated from 0-3Hs collections of fandango maternal mutant and control (FRT42B) embryos using TRIzol Reagent (Invitrogen) following standard protocol. To the RNA samples was performed a DNAse I (Promega) treatment during 30 min at 37C. After, the DNAse was extracted by Phenol-Chloroform extraction, the RNA was pellet by ethanol precipitation and dissolved it in 25 ul of DEPC water. We performed a Bioanalyzer test to analyze the quality and concentration of the samples and finally we brought the volume to 100 ul with water, added 50 ul of 7.5 M NH4OAc, 0.5 ul of glycogen and 250 ul of absolute ethanol.Growth Protocol - Flies were raised using standard techniques. The fandango alleles were isolated from a previously reported maternal screen (Pimenta-Marques et al., 2008).Sample Treatment - Flies were raised using standard techniques.Sequencing - cDNA library was sequenced using the standard Illumina protocolsLibrary Construction - cDNA library was generated using the standard Illumina protocol for RNA-seq (polyA RNAs).OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesUnknownTranscriptomicsGenomicsProteomicsIllumina HiSeq 2000RNA-seq of coding RNADrosophila melanogasterRequirement for highly efficient pre-mRNA splicing during Drosophila early embryonic developmentRui MartinhoLeonardo Guilgur, Pedro Prudêncio, Daniel Sobral, Denisa Liszekova, André Rosa, Rui MartinhoDaniel SobralfalseRNA-seq of coding RNA of fandango, a Drosophila melanogaster mutant affecting splicingTranscriptome sequencing of a D. melanogaster mutant (fandango) that affects splicing, particularly in Early Zygotic genes2014-03-25T00:00:00Z2022-01-26T00:10:39.474Z2022-01-26T00:10:39.474ZE-MTAB-2321ERP004884EFO_0003738