{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Sebastiano Cavallaro"],"study_type":["transcription profiling by array"],"organism":["Homo sapiens"],"species":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-2325"],"description":["Threshold: 1.0 . Logbase: 2 . Technology: Generic.SingleColor.014850. Normalization: Shift to 75 percentile . Baseline Transformation: median of all samples ."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Scaning - Microarrays were scanned at 10-um resolution using a GenePix Personal 4100A microarray scanner and the GenePix Pro 6.0 acquisition and data-extraction software (Molecular Devices, Corp.).","Nucleic Acid Extraction - RNA was extracted with Trizol (Invitrogen) following the manufacturer's standard protocol, followed by confirmation of RNA integrity by agarose gel electrophoresis.","Labeling - Complementary RNAs (cRNAs) labeled with Cy5-CTP (Perkin-Elmer) were synthesized from 1 ug of total RNA of each sample using the Low RNA Input Fluorescent Linear Amplification Kit (Agilent Technologies) following the manufacturer's protocol. A reference cRNA, labeled with Cy3-CTP (Perkin-Elmer), was synthesized from 1 ug of sample D2 RNA. Aliquots (750 ng) of Cy3 and Cy5 labeled cRNA targets were co-hybridized on Whole Human Genome Oligo Microarrays (Agilent Technologies).","Hybridization - Microarray hybridization and washing were performed using reagents and instruments (hybridization chambers and rotating oven) as indicated by the manufacturer (Agilent Technologies)."],"figure_sub":["MIAME Score","Raw Data","Assays and Data","MAGE-TAB Files","Array Designs"],"pubmed_authors":["Sebastiano Cavallaro"],"additional_accession":[]},"is_claimable":false,"name":"Gene expression profiles in motor cortex of Control and Sporadic Amyotrphic Lateral Sclerosis patients","description":"Threshold: 1.0 . Logbase: 2 . Technology: Generic.SingleColor.014850. Normalization: Shift to 75 percentile . Baseline Transformation: median of all samples .","dates":{"release":"2015-02-27T00:00:00Z","modification":"2022-11-17T13:01:47.571Z","creation":"2022-01-31T17:05:19.746Z"},"accession":"E-MTAB-2325","cross_references":{"EFO":["EFO_0002768"]}}