biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsSebastiano Cavallarotranscription profiling by arrayHomo sapiensHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-2325Threshold: 1.0 . Logbase: 2 . Technology: Generic.SingleColor.014850. Normalization: Shift to 75 percentile . Baseline Transformation: median of all samples .biostudies-arrayexpressScaning - Microarrays were scanned at 10-um resolution using a GenePix Personal 4100A microarray scanner and the GenePix Pro 6.0 acquisition and data-extraction software (Molecular Devices, Corp.).Nucleic Acid Extraction - RNA was extracted with Trizol (Invitrogen) following the manufacturer's standard protocol, followed by confirmation of RNA integrity by agarose gel electrophoresis.Labeling - Complementary RNAs (cRNAs) labeled with Cy5-CTP (Perkin-Elmer) were synthesized from 1 ug of total RNA of each sample using the Low RNA Input Fluorescent Linear Amplification Kit (Agilent Technologies) following the manufacturer's protocol. A reference cRNA, labeled with Cy3-CTP (Perkin-Elmer), was synthesized from 1 ug of sample D2 RNA. Aliquots (750 ng) of Cy3 and Cy5 labeled cRNA targets were co-hybridized on Whole Human Genome Oligo Microarrays (Agilent Technologies).Hybridization - Microarray hybridization and washing were performed using reagents and instruments (hybridization chambers and rotating oven) as indicated by the manufacturer (Agilent Technologies).MIAME ScoreRaw DataAssays and DataMAGE-TAB FilesArray DesignsSebastiano CavallarofalseGene expression profiles in motor cortex of Control and Sporadic Amyotrphic Lateral Sclerosis patientsThreshold: 1.0 . Logbase: 2 . Technology: Generic.SingleColor.014850. Normalization: Shift to 75 percentile . Baseline Transformation: median of all samples .2015-02-27T00:00:00Z2022-11-17T13:01:47.571Z2022-01-31T17:05:19.746ZE-MTAB-2325EFO_0002768