{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Jeyaraman Rajaraman"],"organism":["Hordeum vulgare"],"software":["v.10.5.1.1"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-2916"],"description":["To identify the non-host responsive genes, a time-series based global expression profiling was performed in barley using Agilent  chip. Seven-day-old barley plants of cv. Vada were inoculated with spore density of 50-80 conidia mm-2 of Bgh or Bgt, and the abaxial epidermis of inoculated primary leaves or from non-inoculated control leaves was peeled 6, 12, 24 and 74 h after inoculation. Total RNA was extracted by using the RNeasy plant mini kit with on-Column DNase digestion (Qiagen, Hilden, Germany),  and hybridized to a 44K Agilent oligonucleotide custom array of barley."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sample Treatment - Seven-day-old barley plants of cv. Vada were inoculated with spore density of 50-80 conidia mm-2 of Bgh or Bgt, and the abaxial epidermis of inoculated primary leaves or from non-inoculated control leaves was peeled 6, 12, 24 and 74 h after inoculation.","Hybridization - RNA labelling, microarray hybridisation and washing followed the manufacturer's protocols for gene expression arrays (Agilent One-Color Microarray-Based Gene Expression Analysis, version 5.7).","Growth Protocol - For transcript profiling, the barley (Hordeum vulgare subsp. vulgare) cultivar “Vada”,  was used.  Plants of Vada  were grown in  plastic pots (11x14cm diameter) with standard compost soil mixture without fertilization from IPK greenhouse nursery. Approximately 130-150 seeds were sown and seedlings were allowed to grow in a plant  climate chamber (NEMA industrietechnik gmbh) at 19°C with 65% relative humidity (RH) during night and 23°C with 50% RH during day with 16hr of photoperiod.","Scaning - For data extraction, microarray images were imported into Agilent Feature Extraction (FE) software and aligned with the relevant array grid template (020599_D_F_20080612) for barley","Labeling - RNA labelling, microarray hybridisation and washing followed the manufacturer's protocols for gene expression arrays (Agilent One-Color Microarray-Based Gene Expression Analysis, version 5.7).","Nucleic Acid Extraction - Total RNA was extracted by using the RNeasy plant mini kit with on-Column DNase digestion (Qiagen, Hilden, Germany), analysed for quantity and quality by using an Agilent Bioanalyser 2100 (Agilent Technologies, Santa Clara, CA). Seven-day-old barley plants of cv. Vada were inoculated with spore density of 50-80 conidia mm-2 of Bgh or Bgt, and the abaxial epidermis of inoculated primary leaves or from non-inoculated control leaves was peeled 6, 12, 24 and 74 h after inoculation."],"figure_sub":["MIAME Score","Organization","Assays and Data","Processed Data","MAGE-TAB Files","Array Designs"],"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["Agilent G2505B scanner"],"study_type":["transcription profiling by array"],"species":["Hordeum vulgare"],"pubmed_authors":["Jeyaraman Rajaraman","Patrick Schweizer"],"additional_accession":[]},"is_claimable":false,"name":"Identification of candidate non-host resistance genes for powdery mildew in barley","description":"To identify the non-host responsive genes, a time-series based global expression profiling was performed in barley using Agilent  chip. Seven-day-old barley plants of cv. Vada were inoculated with spore density of 50-80 conidia mm-2 of Bgh or Bgt, and the abaxial epidermis of inoculated primary leaves or from non-inoculated control leaves was peeled 6, 12, 24 and 74 h after inoculation. Total RNA was extracted by using the RNeasy plant mini kit with on-Column DNase digestion (Qiagen, Hilden, Germany),  and hybridized to a 44K Agilent oligonucleotide custom array of barley.","dates":{"release":"2014-10-24T00:00:00Z","modification":"2022-03-04T20:05:32.486Z","creation":"2022-03-04T20:05:32.486Z"},"accession":"E-MTAB-2916","cross_references":{"EFO":["EFO_0002768","EFO_0002944","EFO_0003814","EFO_0003813","EFO_0003789","EFO_0003815","EFO_0003969"]}}