{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Anne Francez-Charlot"],"study_type":["transcription profiling by array"],"organism":["Methylobacterium extorquens AM1"],"species":["Methylobacterium extorquens AM1"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-3461"],"description":["Transcriptional profiling of the wild-type strain vs. delta(ecfG1), delta(ecfG2), delta(2) or delta(6) mutant in presence of ethanol 1%"],"repository":["biostudies-arrayexpress"],"pubmed_title":["Diversification and plasticity in the regulatory cascade controlling the general stress response in Alphaproteobacteria"],"sample_protocol":["Labeling - MOgene standard protocol","Growth Protocol - Cultures (20 ml) started from fresh plates were grown for 8-10 h, diluted to an OD600 of 0.04 (50 ml) and incubated ON at 28°C","Hybridization - MOgene standard protocol","Scaning - MOgene standard protocol","Sample Treatment - Cultures were split in two (20 ml each) and supplemented with ethanol (1% final), and further incubated for 20 min at 28°C.","Sample Collection - A solution of 5% phenol in ethanol was added (10% final) and cultures were spun down and the pellets were flash-frozen in liquid nitrogen and kept at -80°C until further processed.","Nucleic Acid Extraction - RNA was extracted with the RNeasy kit (Qiagen) following the manufacturer’s instructions. After purification, RNA samples were treated twice with DNase (Ambion)."],"figure_sub":["MIAME Score","Raw Data","Assays and Data","MAGE-TAB Files","Array Designs"],"pubmed_authors":["Anne Francez-Charlot, Julia Frunzke, Judith Zingg, Andreas Kaczmarczyk and Julia A. Vorholt","Anne Francez-Charlot"],"additional_accession":[]},"is_claimable":false,"name":"Transcriptional profiling by array of wild-type and ecfG mutant strains of Methylobacterium extorquens AM1","description":"Transcriptional profiling of the wild-type strain vs. delta(ecfG1), delta(ecfG2), delta(2) or delta(6) mutant in presence of ethanol 1%","dates":{"release":"2016-02-23T00:00:00Z","modification":"2022-02-04T04:42:10.714Z","creation":"2022-02-04T04:42:10.714Z"},"accession":"E-MTAB-3461","cross_references":{"EFO":["EFO_0002768","EFO_0002944","EFO_0003814","EFO_0003813","EFO_0003789","EFO_0005518","EFO_0003969","EFO_0003815"]}}