biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsAnne Francez-Charlottranscription profiling by arrayMethylobacterium extorquens AM1Methylobacterium extorquens AM1https://www.ebi.ac.uk/biostudies/studies/E-MTAB-3461Transcriptional profiling of the wild-type strain vs. delta(ecfG1), delta(ecfG2), delta(2) or delta(6) mutant in presence of ethanol 1%biostudies-arrayexpressDiversification and plasticity in the regulatory cascade controlling the general stress response in AlphaproteobacteriaLabeling - MOgene standard protocolGrowth Protocol - Cultures (20 ml) started from fresh plates were grown for 8-10 h, diluted to an OD600 of 0.04 (50 ml) and incubated ON at 28°CHybridization - MOgene standard protocolScaning - MOgene standard protocolSample Treatment - Cultures were split in two (20 ml each) and supplemented with ethanol (1% final), and further incubated for 20 min at 28°C.Sample Collection - A solution of 5% phenol in ethanol was added (10% final) and cultures were spun down and the pellets were flash-frozen in liquid nitrogen and kept at -80°C until further processed.Nucleic Acid Extraction - RNA was extracted with the RNeasy kit (Qiagen) following the manufacturer’s instructions. After purification, RNA samples were treated twice with DNase (Ambion).MIAME ScoreRaw DataAssays and DataMAGE-TAB FilesArray DesignsAnne Francez-Charlot, Julia Frunzke, Judith Zingg, Andreas Kaczmarczyk and Julia A. VorholtAnne Francez-CharlotfalseTranscriptional profiling by array of wild-type and ecfG mutant strains of Methylobacterium extorquens AM1Transcriptional profiling of the wild-type strain vs. delta(ecfG1), delta(ecfG2), delta(2) or delta(6) mutant in presence of ethanol 1%2016-02-23T00:00:00Z2022-02-04T04:42:10.714Z2022-02-04T04:42:10.714ZE-MTAB-3461EFO_0002768EFO_0002944EFO_0003814EFO_0003813EFO_0003789EFO_0005518EFO_0003969EFO_0003815