{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":[null],"study_type":["transcription profiling by array"],"organism":["Mus musculus"],"species":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-5382"],"description":["The branchial arches house bilaterally symmetric embryonic vessels, called aortic arches which connect the heart to the embryonic circulation. These undergo a significant remodelling to form the final structure of the great arteries of the neck and head. This study compared the transcriptomes of E11.5 branchial arches and cells isolated from branchial arches and cultured in vitro."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Hybridization - P-AFFY-3 GeneChip hybridization","Growth Protocol - For the cell culture samples, cells were isolated from BA1, BA2 or PBA/OFT tissue pooled from 12-14 embryos and cultured for 24 hours as described (Anderson, M., et al., Differential distribution of the Ca (2+) regulator Pcp4 in the branchial arches is regulated by Hoxa2. PLoS One, 2013. 8(5): p. e63160) prior to RNA extraction.","Nucleic Acid Extraction - For the tissue samples RNA was extracted using Trizol (Invitrogen, 15596-018), pooling 3-5 pairs of BAs for each sample. For the cell culture samples RNA was extracted from cultured cells using RNeasy Micro Kit (Qiagen, 74004).","Scaning - P-AFFY-6 Affymetrix CEL analysis","Labeling - P-AFFY-2 Affymetrix biotin labelling"],"figure_sub":["MIAME Score","Raw Data","Assays and Data","Processed Data","MAGE-TAB Files","Array Designs"],"pubmed_authors":["Leo Zeef"],"data_protocol":["Data Transformation - Microarrays were processed by RMA Bioconductor"],"additional_accession":[]},"is_claimable":false,"name":"Embryonic transcriptional program for remodeling of the heart circulation","description":"The branchial arches house bilaterally symmetric embryonic vessels, called aortic arches which connect the heart to the embryonic circulation. These undergo a significant remodelling to form the final structure of the great arteries of the neck and head. This study compared the transcriptomes of E11.5 branchial arches and cells isolated from branchial arches and cultured in vitro.","dates":{"release":"2026-08-25T00:00:00Z","modification":"2026-08-25T01:00:37.429Z","creation":"2022-01-28T16:41:54.207Z"},"accession":"E-MTAB-5382","cross_references":{"EFO":["EFO_0002768","EFO_0003814","EFO_0002944","EFO_0003813","EFO_0003789","EFO_0003816","EFO_0003815"]}}