{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Owen Robert Dando"],"instrument_platform":["Illumina HiSeq 2500"],"study_type":["RNA-seq of coding RNA"],"organism":["Mus musculus"],"species":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-5688"],"description":["The effect of the Nrf2 activator tBHQ on mouse neurons was studied both when the neurons were cultured alone, and when they were co-cultured with mouse astrocytes."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Growth Protocol - Neurons and astrocytes were cultured as described in Bell et al., Neuronal development is promoted by weakened intrinsic antioxidant defences due to epigenetic repression of Nrf2, Nature Communications 6: 7066 (2015).","Library Construction - RNA-seq libraries were prepared by Edinburgh Genomics using the Illumina TruSeq stranded mRNA-seq kit, according to the manufacturers protocol (Illumina).","Sample Treatment - Pure neuronal cultures or mixed astrocyte/neuronal cultures were treated  tBHQ (10 M) for 8h. At this point RNA was extracted and subject to RNA-seq analysis.","Sequencing - Libraries were sequenced paired-end on an Illumina HiSeq 2500."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Owen Robert Dando"],"additional_accession":[]},"is_claimable":false,"name":"RNA-seq of mouse neurons in mono-culture and in co-culture with astrocytes, with application of Nrf2 activator tBHQ.","description":"The effect of the Nrf2 activator tBHQ on mouse neurons was studied both when the neurons were cultured alone, and when they were co-cultured with mouse astrocytes.","dates":{"release":"2026-04-22T00:00:00Z","modification":"2026-04-22T12:12:42.335Z","creation":"2022-02-03T07:43:59.383Z"},"accession":"E-MTAB-5688","cross_references":{"ENA":["ERP022762"],"EFO":["EFO_0004170","EFO_0003789","EFO_0003738","EFO_0003969","EFO_0004184"]}}