biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsOwen Robert DandoIllumina HiSeq 2500RNA-seq of coding RNAMus musculusMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-5688The effect of the Nrf2 activator tBHQ on mouse neurons was studied both when the neurons were cultured alone, and when they were co-cultured with mouse astrocytes.biostudies-arrayexpressGrowth Protocol - Neurons and astrocytes were cultured as described in Bell et al., Neuronal development is promoted by weakened intrinsic antioxidant defences due to epigenetic repression of Nrf2, Nature Communications 6: 7066 (2015).Library Construction - RNA-seq libraries were prepared by Edinburgh Genomics using the Illumina TruSeq stranded mRNA-seq kit, according to the manufacturers protocol (Illumina).Sample Treatment - Pure neuronal cultures or mixed astrocyte/neuronal cultures were treated tBHQ (10 M) for 8h. At this point RNA was extracted and subject to RNA-seq analysis.Sequencing - Libraries were sequenced paired-end on an Illumina HiSeq 2500.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesOwen Robert DandofalseRNA-seq of mouse neurons in mono-culture and in co-culture with astrocytes, with application of Nrf2 activator tBHQ.The effect of the Nrf2 activator tBHQ on mouse neurons was studied both when the neurons were cultured alone, and when they were co-cultured with mouse astrocytes.2026-04-22T00:00:00Z2026-04-22T12:12:42.335Z2022-02-03T07:43:59.383ZE-MTAB-5688ERP022762EFO_0004170EFO_0003789EFO_0003738EFO_0003969EFO_0004184