biostudies-arrayexpressManuel MartinezHordeum vulgare subsp. vulgarehttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-6565Drought and herbivores are main threats to crop production. Barley plants were subjected to dehydration, spider mite attack or to a combination of both stresses. RNA-seq analyses were done to know how individual and double abiotic-biotic stresses promote changes in the transcriptome.biostudies-arrayexpressSample Collection - Leaves were harvested after 7 days of treatment. Samples were frozen into liquid nitrogen and stored at -80ºC.Sample Treatment - For spider mite infestation, each plant was infested with 20 adult female mites adapted to barley. The infestation was performed by placing a barley leaf with the mites on the experimental plant leaves. A falcon tube with holes was used to help maintaining the leaves together. Barley plants were confined in pots with plastic cylinders covered on top by nylon nets to avoid dispersion of mites. The same isolation system was applied to control and dehydration stressed plants. For dehydration treatments, pots were placed over Petri plastic plates to individualize watering. Plants were subjected to dehydration stress imposed by continuous water deprivation. Controls were watered to maintain soil moisture at 70% (considered as optimal watering). In addition, both stresses were combined.Nucleic Acid Extraction - Total RNA was extracted from frozen barley leaves by the phenol/chloroform method, followed by precipitation with 8 M LiCl and digested with DNase (Promega).Sequencing - The cDNA libraries were sequenced using the Illumina HiSeq2000 platform by the Beijing Genomics Institute (BGI).Library Construction - Using poly-T oligo-attached magnetic beads, mRNAs were purified from the total RNA. Then, the mRNAs were fragmented and cDNA was synthesized using random hexamer-primers, DNA polymerase I and RNase H. The double-stranded cDNAs were purified with magnetic beads and ligated to adaptors for Illumina sequencing. The quality and quantity of the library was verified using an Agilent 2100 Bioanalyzer and an ABI StepOnePlus Real-Time PCR system, respectively.Growth Protocol - Barley plants of Hordeum vulgare cv. Golden Promise were used. Grains were germinated in soil and grown at 22ºC under a 16 h light/8 h darkness photoperiod for 7 days in Sanyo MLR-350-H chambers. A colony of the two-spotted spider mite Tetranychus urticae London strain (Acari: Tetranychidae) was maintained on beans in a Sanyo MLR-350-H growth chamber at 25ºC under a 16 h light/8 h darkness photoperiod. This colony was transferred to barley where it was maintained under the same conditions for more than 30 generations to ensure mite adaptation to the host.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesUnknownTranscriptomicsGenomicsProteomicsIllumina HiSeq 2000RNA-seq of coding RNAHordeum vulgare subsp. vulgareDehydration stress contributes to enhance plant defense response and mite performance on barleyManuel MartinezM. Estrella Santamaria, Isabel Diaz, Manuel MartinezfalseRNA-seq of barley lines after dehydration, spider mite or combined stress.Drought and herbivores are main threats to crop production. Barley plants were subjected to dehydration, spider mite attack or to a combination of both stresses. RNA-seq analyses were done to know how individual and double abiotic-biotic stresses promote changes in the transcriptome.2018-04-30T00:00:00Z2022-03-04T19:22:19.042Z2022-03-04T19:22:19.042ZE-MTAB-6565ERP107297EFO_0002944EFO_0004170EFO_0003789EFO_0005518EFO_0003738EFO_0003969EFO_0004184