{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Keiichi Mochida"],"organism":["Euglena agilis"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-7293"],"description":["To analyze the transcriptome after heat stress in Euglena agilis,  we analyzed a series of RNA-seq data from E. agilis cells grown under control (23°C) and heat stress (35°C) conditions at 2 time-points (at 8 and 24 h of heat stress exposure)."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sample Treatment - We cultured E. agilis in mAC media at 23°C for more than 3 days and transferred the cells to a temperature of 35°C and incubated them for 8 and 24 h for mRNA preparation.","Sample Collection - The culture was maintained in 100-mL test tubes containing 50 mL of media with aeration (50 mL/min) and constant illumination (100 μmol photon/m2s) in four biological replicates.","Nucleic Acid Extraction - Total RNA was extracted from the cells cultured at 23°C (control) and 35°C for 8 and 24 h using NucleoSpin RNA (Macherey-Nagel).","Growth Protocol - We cultured E. agilis in mAC media at 23°C for more than 3 days and transferred the cells to a temperature of 35°C and incubated them for 8 and 24 h for mRNA preparation.","Library Construction - Sequencing libraries were constructed using a TruSeq Stranded mRNA Sample Preparation Kit (Illumina, Inc.).","Sequencing - The sequencing libraries were sequenced using a Hiseq 4000 with a TruSeq SBS Kit v2 – HS (Illumina, Inc.) by the paired-end sequencing method for sequences 100 bp in length."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","Processed Data","MAGE-TAB Files"],"data_protocol":["Sequence Alignment - The quality-checked transcriptome sequencing reads from the heat stress treated cells of E. agilis, were mapped to the E. agilis genome using HISAT2 (ver. 2.0.5) with the parameter setting of --pen-noncansplice = 0 -no-softclip.","Data Transformation - Normalized read counts for the E. agilis genes were computed by using the featureCounts program (v. 1.5.0-p3) with default parameters."],"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["Illumina HiSeq 4000"],"study_type":["RNA-seq of coding RNA"],"species":["Euglena agilis"],"pubmed_authors":["Keiichi Mochida"],"additional_accession":[]},"is_claimable":false,"name":"Heat stress transcriptome analysis in Euglena agilis","description":"To analyze the transcriptome after heat stress in Euglena agilis,  we analyzed a series of RNA-seq data from E. agilis cells grown under control (23°C) and heat stress (35°C) conditions at 2 time-points (at 8 and 24 h of heat stress exposure).","dates":{"release":"2025-09-01T00:00:00Z","modification":"2025-09-01T01:02:25.111Z","creation":"2022-01-28T21:38:51.068Z"},"accession":"E-MTAB-7293","cross_references":{"ENA":["ERP111434"],"EFO":["EFO_0002944","EFO_0004170","EFO_0003789","EFO_0004917","EFO_0003816","EFO_0005518","EFO_0003738","EFO_0004184","EFO_0003969"]}}