biostudies-arrayexpressKeiichi MochidaEuglena agilishttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-7293To analyze the transcriptome after heat stress in Euglena agilis, we analyzed a series of RNA-seq data from E. agilis cells grown under control (23°C) and heat stress (35°C) conditions at 2 time-points (at 8 and 24 h of heat stress exposure).biostudies-arrayexpressSample Treatment - We cultured E. agilis in mAC media at 23°C for more than 3 days and transferred the cells to a temperature of 35°C and incubated them for 8 and 24 h for mRNA preparation.Sample Collection - The culture was maintained in 100-mL test tubes containing 50 mL of media with aeration (50 mL/min) and constant illumination (100 μmol photon/m2s) in four biological replicates.Nucleic Acid Extraction - Total RNA was extracted from the cells cultured at 23°C (control) and 35°C for 8 and 24 h using NucleoSpin RNA (Macherey-Nagel).Growth Protocol - We cultured E. agilis in mAC media at 23°C for more than 3 days and transferred the cells to a temperature of 35°C and incubated them for 8 and 24 h for mRNA preparation.Library Construction - Sequencing libraries were constructed using a TruSeq Stranded mRNA Sample Preparation Kit (Illumina, Inc.).Sequencing - The sequencing libraries were sequenced using a Hiseq 4000 with a TruSeq SBS Kit v2 – HS (Illumina, Inc.) by the paired-end sequencing method for sequences 100 bp in length.OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesSequence Alignment - The quality-checked transcriptome sequencing reads from the heat stress treated cells of E. agilis, were mapped to the E. agilis genome using HISAT2 (ver. 2.0.5) with the parameter setting of --pen-noncansplice = 0 -no-softclip.Data Transformation - Normalized read counts for the E. agilis genes were computed by using the featureCounts program (v. 1.5.0-p3) with default parameters.MetabolomicsUnknownTranscriptomicsGenomicsProteomicsIllumina HiSeq 4000RNA-seq of coding RNAEuglena agilisKeiichi MochidafalseHeat stress transcriptome analysis in Euglena agilisTo analyze the transcriptome after heat stress in Euglena agilis, we analyzed a series of RNA-seq data from E. agilis cells grown under control (23°C) and heat stress (35°C) conditions at 2 time-points (at 8 and 24 h of heat stress exposure).2025-09-01T00:00:00Z2025-09-01T01:02:25.111Z2022-01-28T21:38:51.068ZE-MTAB-7293ERP111434EFO_0002944EFO_0004170EFO_0003789EFO_0004917EFO_0003816EFO_0005518EFO_0003738EFO_0004184EFO_0003969