{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Leo Eberl"],"organism":["Burkholderia cenocepacia"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-732"],"description":["The purpose of this experiment was to determine differences in expression between wild-type B. cenocepacia H111 and two Tn5 mutants, R12 and R33, isolated during a Tn-mutagenesis screen for reduced pathogenicity."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Growth Protocol - Cells were grown  in LB at 37°C with shaking, to an OD600 of 2.7"],"figure_sub":["MIAME Score","Organization","Assays and Data","Processed Data","MAGE-TAB Files","Array Designs"],"data_protocol":["Image Adquisition - Scan the washed and dried arrays as soon as possible using amicroarray scanner (G2565 BA, Agilent) and the Scan Control softwareversion A.7.0.3 Feb 2007 (Agilent).- Place the slides in the slide holder so that the numeric barcode isvisible.- Place the slides in the scanner, open the scan control software.- Scanner settings: scan region 61 x 21.6 mmscan resolution 5?m- Select Extended Dynamic Range with XDR Hi 100% and XDR Lo 10%(100% and 10% PMT gain).- Start the scan.- Every slide is scanned twice and two image files are generated: onewith the suffix 'H' and one with the suffix 'L'.- View the scanned images with the Feature Extraction software(Agilent)."],"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"study_type":["transcription profiling by array"],"species":["Burkholderia cenocepacia"],"pubmed_title":["Exposing the Third Chromosome of Burkholderia cepacia complex Strains as a Virulence Plasmid"],"pubmed_authors":["Leo Eberl","K. Agnoli, S. Schwager, S. Uehlinger, A. Vergunst, D.F. Viteri, D.T. Nguyen, P.A. Sokol, A. Carlier and L. Eberl"],"additional_accession":[]},"is_claimable":false,"name":"Comparison of H111 c3-null mutants R12 and R33 to wild-type","description":"The purpose of this experiment was to determine differences in expression between wild-type B. cenocepacia H111 and two Tn5 mutants, R12 and R33, isolated during a Tn-mutagenesis screen for reduced pathogenicity.","dates":{"release":"2012-07-11T00:00:00Z","modification":"2022-03-07T00:26:25.901Z","creation":"2022-03-07T00:26:25.901Z"},"accession":"E-MTAB-732","cross_references":{"EFO":["EFO_0002768"]}}