biostudies-arrayexpressLeo EberlBurkholderia cenocepaciahttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-732The purpose of this experiment was to determine differences in expression between wild-type B. cenocepacia H111 and two Tn5 mutants, R12 and R33, isolated during a Tn-mutagenesis screen for reduced pathogenicity.biostudies-arrayexpressGrowth Protocol - Cells were grown in LB at 37°C with shaking, to an OD600 of 2.7MIAME ScoreOrganizationAssays and DataProcessed DataMAGE-TAB FilesArray DesignsImage Adquisition - Scan the washed and dried arrays as soon as possible using amicroarray scanner (G2565 BA, Agilent) and the Scan Control softwareversion A.7.0.3 Feb 2007 (Agilent).- Place the slides in the slide holder so that the numeric barcode isvisible.- Place the slides in the scanner, open the scan control software.- Scanner settings: scan region 61 x 21.6 mmscan resolution 5?m- Select Extended Dynamic Range with XDR Hi 100% and XDR Lo 10%(100% and 10% PMT gain).- Start the scan.- Every slide is scanned twice and two image files are generated: onewith the suffix 'H' and one with the suffix 'L'.- View the scanned images with the Feature Extraction software(Agilent).UnknownTranscriptomicsGenomicsProteomicstranscription profiling by arrayBurkholderia cenocepaciaExposing the Third Chromosome of Burkholderia cepacia complex Strains as a Virulence PlasmidLeo EberlK. Agnoli, S. Schwager, S. Uehlinger, A. Vergunst, D.F. Viteri, D.T. Nguyen, P.A. Sokol, A. Carlier and L. EberlfalseComparison of H111 c3-null mutants R12 and R33 to wild-typeThe purpose of this experiment was to determine differences in expression between wild-type B. cenocepacia H111 and two Tn5 mutants, R12 and R33, isolated during a Tn-mutagenesis screen for reduced pathogenicity.2012-07-11T00:00:00Z2022-03-07T00:26:25.901Z2022-03-07T00:26:25.901ZE-MTAB-732EFO_0002768