biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsHaico van AttikumNextSeq 500RNA-seq of coding RNASaccharomyces cerevisiaeSaccharomyces cerevisiaehttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-7471RNA-seq in wild-type yeast cells to determine the effect of crotonate treatment on the transcriptome. Three independent yeast colonies were used for inoculation. Overnight cultures were diluted in fresh medium with or without 10 mM sodium crotonate in the presence of 0.8 M sorbitol. After 3.5 hours cells were in early log-phase (1 × 10^7 cells/ml) and harvested by centrifugation. Total RNA was isolated using the RNeasyMini kit and treated with the RNase-Free DNase Set to remove any contaminating genomic DNA. Library preparations were performed with the TruSeq Stranded mRNA Library kit (Illumina). Three independent biological replicates for each condition were subjected to RNA-seq analysis using an Illumina NextSeq500 with a 75 bp single read flowcell.biostudies-arrayexpressSequencing - Three independent biological replicates for each condition were subjected to RNA-seq analysis using an Illumina NextSeq500 with a 75 bp single read flowcell.Nucleic Acid Extraction - Total RNA was isolated using the RNeasyMini kit (Qiagen) and treated with the RNase-Free DNase Set (Qiagen) to remove any contaminating genomic DNA.Library Construction - Library preparations were performed with the TruSeq Stranded mRNA Library kit (Illumina).Sample Collection - Three independent yeast colonies were used for inoculation. Overnight cultures were diluted in fresh medium with or without 10 mM sodium crotonate (Sigma-Aldrich) in the presence of 0.8 M sorbitol (Sigma-Aldrich). After 3.5 hours cells were in early log-phase (1 × 10^7 cells/ml) and harvested by centrifugation.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesHaico van AttikumfalseRNA-seq in wild-type Saccharomyces cerevisiae S288c cells to determine the effect of sodium crotonate treatment on the transcriptomeRNA-seq in wild-type yeast cells to determine the effect of crotonate treatment on the transcriptome. Three independent yeast colonies were used for inoculation. Overnight cultures were diluted in fresh medium with or without 10 mM sodium crotonate in the presence of 0.8 M sorbitol. After 3.5 hours cells were in early log-phase (1 × 10^7 cells/ml) and harvested by centrifugation. Total RNA was isolated using the RNeasyMini kit and treated with the RNase-Free DNase Set to remove any contaminating genomic DNA. Library preparations were performed with the TruSeq Stranded mRNA Library kit (Illumina). Three independent biological replicates for each condition were subjected to RNA-seq analysis using an Illumina NextSeq500 with a 75 bp single read flowcell.2019-11-06T00:00:00Z2022-02-03T07:32:53.432Z2022-02-03T07:32:53.432ZE-MTAB-7471ERP112286EFO_0002944EFO_0004170EFO_0005518EFO_0003738EFO_0004184