{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Christine Clayton"],"instrument_platform":["NextSeq 500"],"study_type":["Cell line - High-throughput sequencing"],"organism":["Trypanosoma brucei"],"species":["Trypanosoma brucei"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-7996"],"description":["ZC3H20 and ZC3H21 were targeted by stem-loop RNAi using a shared region. Induction was for 36h."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Library Construction - rRNAs were depleted with anti-sence oligos and RNaseH. Libraries were made according to the manuscript.","Sample Collection - The cells were centrifuged and resuspended with Trizol.","Nucleic Acid Extraction - Trizol. according the manuscript.","Sample Treatment - Cells were treated with tet for 36h, or not treated.","Sequencing - According to the manuscript."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Christine Clayton"],"additional_accession":[]},"is_claimable":false,"name":"RNAi targeting both ZC3H20 and ZC3H21 in procyclic-form Lister427 Trypanosoma brucei","description":"ZC3H20 and ZC3H21 were targeted by stem-loop RNAi using a shared region. Induction was for 36h.","dates":{"release":"2019-06-02T00:00:00Z","modification":"2022-01-28T20:39:00.52Z","creation":"2022-01-28T20:39:00.52Z"},"accession":"E-MTAB-7996","cross_references":{"ENA":["ERP115437"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005518","EFO_0003969","EFO_0004184"]}}