biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsChristine ClaytonNextSeq 500Cell line - High-throughput sequencingTrypanosoma bruceiTrypanosoma bruceihttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-7996ZC3H20 and ZC3H21 were targeted by stem-loop RNAi using a shared region. Induction was for 36h.biostudies-arrayexpressLibrary Construction - rRNAs were depleted with anti-sence oligos and RNaseH. Libraries were made according to the manuscript.Sample Collection - The cells were centrifuged and resuspended with Trizol.Nucleic Acid Extraction - Trizol. according the manuscript.Sample Treatment - Cells were treated with tet for 36h, or not treated.Sequencing - According to the manuscript.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesChristine ClaytonfalseRNAi targeting both ZC3H20 and ZC3H21 in procyclic-form Lister427 Trypanosoma bruceiZC3H20 and ZC3H21 were targeted by stem-loop RNAi using a shared region. Induction was for 36h.2019-06-02T00:00:00Z2022-01-28T20:39:00.52Z2022-01-28T20:39:00.52ZE-MTAB-7996ERP115437EFO_0002944EFO_0004170EFO_0005518EFO_0003969EFO_0004184