{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Valerio Pazienza"],"organism":["human gut metagenome"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-8136"],"description":["In this study we investigated whether gut microbiota profile of Italian healthy volunteers could differ based on their geaographical origin. To this purpose, fecal samples were collected from 31 healthy individuals living in 3 different italian regions (Lombardy, North; Lazio, Center; Apulia, South) and their respective microbiota profiles were analyzed employing 16S metagenomic sequencing method. This study identifies differences in the gut microbiota content and richness among individuals with the same ethnicity coming from three different Italian regions."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Library Construction - The V3–V4 hypervariable region of the bacterial 16S  rRNA gene was amplified from total DNA according to the Illumina 16S Metagenomic Sequencing Library Preparation instructions, as previously described in Diamanti-Picchianti A, et al (Int J Mol Med ). In summary, the V3-V4 amplicon was obtained by PCR with universal primers, followed by purification. A second PCR was then performed to barcode the libraries using the Illumina dual-index system. Following a second step of purification, libraries were diluted to 4nM and pooled.","Sample Collection - Each participant provided the fresh stool sample in a collection tube filled with a DNA stabilization buffer (Canvax Biotech).","Nucleic Acid Extraction - DNA microbial extraction was performed using the QIAamp DNA Stool Mini Kit (Qiagen) starting from 250 µl of each sample according to the manufacturer’s protocol. After assessing DNA concentration and purity, samples were stored at -80° until processing.","Sequencing - Paired-end sequencing (2 x 300 cycles) was carried out on an Illumina MiSeq device (Illumina Inc., San Diego, CA, USA) according to the manufacturer's specifications."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["Illumina MiSeq"],"study_type":["DNA-seq"],"species":["human gut metagenome"],"pubmed_title":["Gut microbiota profile differs among individuals depending on their region of origin: an Italian Pilot Study"],"pubmed_authors":["Panebianco C, Fontana A, Picchianti-Diamanti A, Laganà B, Cavalieri D, Potenza A, Pracella R, Binda E, Copetti M, Pazienza V.","Valerio Pazienza"],"additional_accession":[]},"is_claimable":false,"name":"16S sequencing of gut microbiota in healthy individuals from different regions","description":"In this study we investigated whether gut microbiota profile of Italian healthy volunteers could differ based on their geaographical origin. To this purpose, fecal samples were collected from 31 healthy individuals living in 3 different italian regions (Lombardy, North; Lazio, Center; Apulia, South) and their respective microbiota profiles were analyzed employing 16S metagenomic sequencing method. This study identifies differences in the gut microbiota content and richness among individuals with the same ethnicity coming from three different Italian regions.","dates":{"release":"2026-01-08T00:00:00Z","modification":"2026-01-08T02:02:04.171Z","creation":"2022-01-31T16:50:31.422Z"},"accession":"E-MTAB-8136","cross_references":{"ENA":["ERP116228"],"EFO":["EFO_0002944","EFO_0004170","EFO_0002693","EFO_0005518","EFO_0004184"]}}