{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Mai Lunde"],"disease":["oral submucous fibrosis"],"study_type":["comparative genomic hybridization by array"],"organism":["Homo sapiens"],"species":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-819"],"description":["In Asia, oral cancer (OC) and oral submucous fibrosis (OSF) constitute major health problems linked to use of betel quid. This work performed CGH genome-wide analysis of OC (12 from India, 12 from Sri Lanka) and OSF (6 from India) cases with normal controls."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Labeling - 500 ng of total DNA were digested overnight at 37C with DpnII (New England Biolabs, Ipswich, MA; USA), and tumor DNA was labeled with Cy3-dCTP and control DNA (human Universal Reference DNA) with Cy5-cCTP (NEN Life Science Products Inc., Boston, MA, USA) by random priming. The labeled DNA samples were combined and mixed with 135 ug human Cot-1 DNA (Roche Diagnostics Corp., Indianapolis, IN, USA).","Hybridization - Hybridization was performed using an automated hybridization station GeneTAC/HybArray (Genomic Solutions, Ann Arbor, MI, USA) with agitation for 42 hours at 37C, and arrays were scanned using an Agilent G2565B scanner (Agilent Technologies Inc, Santa Clara, CA, USA). The method has been described in detail by Meza-Zepeda et al. Cancer Res 2006, 66(18):8984-8993."],"figure_sub":["MIAME Score","Raw Data","Organization","Assays and Data","Processed Data","MAGE-TAB Files","Array Designs"],"pubmed_authors":["Ravi Mehrotra","Endre Vasstrand","Saman Warnakulasuriya","Jussi Laranne","Salah Ibrahim","Mai Lunde","Eric Roman1"],"additional_accession":[]},"is_claimable":false,"name":"OSCC CGH SriLanka vs India","description":"In Asia, oral cancer (OC) and oral submucous fibrosis (OSF) constitute major health problems linked to use of betel quid. This work performed CGH genome-wide analysis of OC (12 from India, 12 from Sri Lanka) and OSF (6 from India) cases with normal controls.","dates":{"release":"2011-11-30T00:00:00Z","modification":"2022-02-08T00:14:28.236Z","creation":"2022-02-08T00:14:28.236Z"},"accession":"E-MTAB-819","cross_references":{"EFO":["EFO_0000749"]}}