{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Paloma Bonato"],"instrument_platform":["Ion Torrent Proton"],"study_type":["RNA-seq of total RNA"],"organism":["Herbaspirillum seropedicae"],"species":["Herbaspirillum seropedicae"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-8268"],"description":["H. seropedicae A01 mutant grown on three different nitrogen conditions: nitrogen limiting, ammonium shock and nitrate shock."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Library Construction - cDNA libraries were constructed using Ion Total RNASeq kit v2 (Thermo Fisher Scientific).","Growth Protocol - Cells were cultivated in NFbHP-malate supplemented with 1 mM NH4Cl, at 30C and 250 rpm. When cells reached OD600 = 0.4, cells were collected and stored in RNAlater. Additionally, the remaining cells were incubated for a further 30 minutes after addition of 10 mM NH4Cl (Ammonium shock) or 10 mM KNO3 (Nitrate shock). After incubation, cells were collected and stored in RNAlater.","Nucleic Acid Extraction - RNA was extracted using Trizol (Thermo Fisher Scientific), and contaminant DNA was removed using TURBO DNase (Thermo Fisher Scientific). rRNA was removed using Ribo-Zero rRNA Removal kit (Gram-negative bacteria) (Illumina).","Sample Collection - Cells were collected and stored in RNAlater stabilization solution (Thermo Fisher Scientific).","Sequencing - cDNA libraries were sequenced in Ion Proton Sequencer (Thermo Fisher Scientific)"],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Paloma Bonato"],"additional_accession":[]},"is_claimable":false,"name":"Herbaspirillum seropedicae A01 mutant grown on three nitrogen conditions: Ammonium limiting, Ammonium shock and Nitrate shock","description":"H. seropedicae A01 mutant grown on three different nitrogen conditions: nitrogen limiting, ammonium shock and nitrate shock.","dates":{"release":"2026-05-30T00:00:00Z","modification":"2026-05-30T01:01:22.648Z","creation":"2022-02-07T23:18:25.982Z"},"accession":"E-MTAB-8268","cross_references":{"ENA":["ERP116968"],"EFO":["EFO_0002944","EFO_0004170","EFO_0009653","EFO_0003789","EFO_0005518","EFO_0004184"]}}