biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsPaloma BonatoIon Torrent ProtonRNA-seq of total RNAHerbaspirillum seropedicaeHerbaspirillum seropedicaehttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-8268H. seropedicae A01 mutant grown on three different nitrogen conditions: nitrogen limiting, ammonium shock and nitrate shock.biostudies-arrayexpressLibrary Construction - cDNA libraries were constructed using Ion Total RNASeq kit v2 (Thermo Fisher Scientific).Growth Protocol - Cells were cultivated in NFbHP-malate supplemented with 1 mM NH4Cl, at 30C and 250 rpm. When cells reached OD600 = 0.4, cells were collected and stored in RNAlater. Additionally, the remaining cells were incubated for a further 30 minutes after addition of 10 mM NH4Cl (Ammonium shock) or 10 mM KNO3 (Nitrate shock). After incubation, cells were collected and stored in RNAlater.Nucleic Acid Extraction - RNA was extracted using Trizol (Thermo Fisher Scientific), and contaminant DNA was removed using TURBO DNase (Thermo Fisher Scientific). rRNA was removed using Ribo-Zero rRNA Removal kit (Gram-negative bacteria) (Illumina).Sample Collection - Cells were collected and stored in RNAlater stabilization solution (Thermo Fisher Scientific).Sequencing - cDNA libraries were sequenced in Ion Proton Sequencer (Thermo Fisher Scientific)OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesPaloma BonatofalseHerbaspirillum seropedicae A01 mutant grown on three nitrogen conditions: Ammonium limiting, Ammonium shock and Nitrate shockH. seropedicae A01 mutant grown on three different nitrogen conditions: nitrogen limiting, ammonium shock and nitrate shock.2026-05-30T00:00:00Z2026-05-30T01:01:22.648Z2022-02-07T23:18:25.982ZE-MTAB-8268ERP116968EFO_0002944EFO_0004170EFO_0009653EFO_0003789EFO_0005518EFO_0004184