{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Stefano Biffo"],"instrument_platform":["Illumina HiSeq 2500"],"study_type":["RNA-seq of coding RNA"],"organism":["Homo sapiens"],"species":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-8496"],"description":["Total RNAs were isolated from Delta47 human myeloma cells expressing either the FNDC3A protein or a Mock control and sequenced. The Delta47 cell line lacks FNDC3A expression, so the purpose of the experiment was to quantify the differentially-abundant mRNAs in the two cell lines."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Nucleic Acid Extraction - Total RNA from control delta47 cells and delta47 cells expressing FNDC3A were isolated using the RNeasy kit (Qiagen)","Sample Collection - Delta47 cell lines were grown in RPMI 1640 medium containing 100 U/ml penicillin and 100 microg/ml streptomycin supplemented with 20% FBS and 1% glutamine.","Library Construction - Random primed cDNA libraries were constructed from 100 ng of total RNA.","Sequencing - A paired-end (2×125) run was performed with 50 million reads per sample."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Stefano Biffo","Nicola Manfrini","Riccardo Rossi"],"additional_accession":[]},"is_claimable":false,"name":"RNA-seq of Delta47 human myeloma cells expressing either the FNDC3A protein or a Mock control","description":"Total RNAs were isolated from Delta47 human myeloma cells expressing either the FNDC3A protein or a Mock control and sequenced. The Delta47 cell line lacks FNDC3A expression, so the purpose of the experiment was to quantify the differentially-abundant mRNAs in the two cell lines.","dates":{"release":"2020-10-22T00:00:00Z","modification":"2022-02-04T05:39:23.123Z","creation":"2022-02-04T05:39:23.123Z"},"accession":"E-MTAB-8496","cross_references":{"ENA":["ERP118228"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005518","EFO_0003738","EFO_0004184"]}}