biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsStefano BiffoIllumina HiSeq 2500RNA-seq of coding RNAHomo sapiensHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-8496Total RNAs were isolated from Delta47 human myeloma cells expressing either the FNDC3A protein or a Mock control and sequenced. The Delta47 cell line lacks FNDC3A expression, so the purpose of the experiment was to quantify the differentially-abundant mRNAs in the two cell lines.biostudies-arrayexpressNucleic Acid Extraction - Total RNA from control delta47 cells and delta47 cells expressing FNDC3A were isolated using the RNeasy kit (Qiagen)Sample Collection - Delta47 cell lines were grown in RPMI 1640 medium containing 100 U/ml penicillin and 100 microg/ml streptomycin supplemented with 20% FBS and 1% glutamine.Library Construction - Random primed cDNA libraries were constructed from 100 ng of total RNA.Sequencing - A paired-end (2×125) run was performed with 50 million reads per sample.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesStefano BiffoNicola ManfriniRiccardo RossifalseRNA-seq of Delta47 human myeloma cells expressing either the FNDC3A protein or a Mock controlTotal RNAs were isolated from Delta47 human myeloma cells expressing either the FNDC3A protein or a Mock control and sequenced. The Delta47 cell line lacks FNDC3A expression, so the purpose of the experiment was to quantify the differentially-abundant mRNAs in the two cell lines.2020-10-22T00:00:00Z2022-02-04T05:39:23.123Z2022-02-04T05:39:23.123ZE-MTAB-8496ERP118228EFO_0002944EFO_0004170EFO_0005518EFO_0003738EFO_0004184