biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsShinkichi Ishikawatranscription profiling by arrayHomo sapiensHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-8920The current study was performed for analysis of the biological effects of vapor from novel tobacco vapor product in comparison with 3R4F cigarette smoke. This study was performed using a three-dimensional culture system composed of an air-liquid interface culture of primary normal human bronchial epithelial cells (MucilAir). The MucilAir tissues were subjected to 17 days of exposure to the aqueous extract of novel tobacco product vapor or 3R4F cigarette smoke. The number of differentially expressed genes increased in MucilAir tissues exposed to aqueous extract of each test product dependent on exposure duration. The number of differentially expressed genes was lower in the tissues exposed to aqueous extract of novel tobacco product vapor compared to the tissues exposed to aqueous extract of 3R4F cigarette smoke.biostudies-arrayexpressHybridization - The biotin-labeled RNA was Hybridized on Affymetrix GeneChip Human Genome U133 Plus 2.0 Array using GeneChip® Hybridization, Wash and Stain Kit.Scaning - Command Console Software (Affymetrix) was used to automatically grid the DAT files and create the CEL files (probe cell intensity data).Labeling - Biotinylated cRNA was generated using GeneChip® 3′IVT PLUS Reagent Kit.Nucleic Acid Extraction - Total RNA was isolated and purified using the Qiagen RNeasy® Mini Kit (Qiagen, Hilden, Germany).Sample Collection - The organotypic human bronchial epithelial cultures MucilAir (product code EP01, Epithelix Sarl, Geneve, Switzerland) were used in this study. The cells were incubated at 37°C with a 5% CO2 atmosphere for acclimation for 10 days before use. Then the cells were cultivated in the medium for exposure; 600 μL of the MucilAir culture medium (Epithelix Sarl) mixed with 100 μL of the aqueous aerosol extract of each test product. The medium for exposure was changed every 2 or 3 days during the 17 days of exposure.The aqueous aerosol extract of each test product was prepared by bubbling the smoking machine generated aerosol into the 15 mL of ice-cold Dulbecco’s modified Eagle’s medium. The international organization for standardization intensive smoking regime (55-mL puffs of 2 sec in duration every 30 sec) was used for generation of the 3R4F cigarette smoke. The international organization for standardization standard regime for vapor products (55-mL puffs of 3 sec in duration every 30 sec) was used for generation of the novel tobacco product vapor. The concentration of aqueous aerosol extract exposed of tissues was expressed with the number of puffs bubbled into the medium (0.025, 0.05, 0.1, and 0.2 puffs/mL for 3R4F cigarette smoke and 0.25. 0.5, 1.0, and 2.0 puffs/mL for novel tobacco product vapor). The tissues collected at three different time points (following 1, 8, and 17 days of exposure) were subjected to mRNA extraction for transcriptomic analysis.MIAME ScoreRaw DataOrganizationAssays and DataMAGE-TAB FilesArray DesignsShinkichi IshikawafalseOrganotypic human bronchial epithelial cultures (MucilAir) exposed to aqueous aerosol extracts of the 3R4F cigarette smoke and a novel tobacco product vaporThe current study was performed for analysis of the biological effects of vapor from novel tobacco vapor product in comparison with 3R4F cigarette smoke. This study was performed using a three-dimensional culture system composed of an air-liquid interface culture of primary normal human bronchial epithelial cells (MucilAir). The MucilAir tissues were subjected to 17 days of exposure to the aqueous extract of novel tobacco product vapor or 3R4F cigarette smoke. The number of differentially expressed genes increased in MucilAir tissues exposed to aqueous extract of each test product dependent on exposure duration. The number of differentially expressed genes was lower in the tissues exposed to aqueous extract of novel tobacco product vapor compared to the tissues exposed to aqueous extract of 3R4F cigarette smoke.2020-04-15T00:00:00Z2022-03-10T07:58:57.064Z2022-03-10T07:58:57.064ZE-MTAB-8920EFO_0002768EFO_0002944EFO_0003814EFO_0003813EFO_0005518EFO_0003815